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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PARP11 Polyclonal antibody (16692-1-AP)

    WB analysis using 16692-1-AP (same clone as 16692-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16692-1-AP (PARP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16692-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PARP11 Polyclonal antibody (16692-1-AP)

    WB analysis of HeLa using 16692-1-AP (same clone as 16692-1-PBS)

    WB result of PARP11 antibody (16692-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PARP11 transfected HeLa cells. This data was developed using the same antibody clone with 16692-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PARP11 Polyclonal antibody (16692-1-AP)

    WB analysis using 16692-1-AP (same clone as 16692-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16692-1-AP (PARP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16692-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using PARP11 Polyclonal antibody (16692-1-AP)

    WB analysis of PC-3 using 16692-1-AP (same clone as 16692-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16692-1-AP (PARP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16692-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PARP11 Polyclonal antibody, PBS Only

PARP11 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 16692-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

ADP-ribosyltransferase diphtheria toxin-like 11, ARTD11, C12orf6, EC:2.4.2.-, MIB006

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16692-1-PBS targets PARP11 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10053

Product name: Recombinant human PARP11 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-331 aa of BC017569

Sequence: MFHKAEELFSKTTNNEVDDMDTSDTQWGWFYLAECGKWHMFQPDTNSQCSVSSEDIEKSFKTNPCGSISFTTSKFSYKIDFAEMKQMNLTTGKQRLIKRAPFSISAFSYICENEAIPMPPHWENVNTQVPYQLIPLHNQTHEYNEVANLFGKTMDRNRIKRIQRIQNLDLWEFFCRKKAQLKKKRGVPQINEQMLFHGTSSEFVEAICIHNFDWRINGIHGAVFGKGTYFARDAAYSSRFCKDDIKHGNTFQIHGVSLQQRHLFRTYKSMFLARVLIGDYINGDSKYMRPPSKDGSYVNLYDSCVDDTWNPKIFVVFDANQIYPEYLIDFH

相同性解析による交差性が予測される生物種
Full Name poly (ADP-ribose) polymerase family, member 11
Calculated molecular weight 331 aa, 39 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC017569
Gene Symbol PARP11
Gene ID (NCBI) 57097
RRIDAB_2158504
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR21
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PARP11, also named as ARTD11, belongs to the  ARTD/PARP family. PARP11 is a mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins. PARP11 plays a role in nuclear envelope stability and nuclear remodeling during spermiogenesis (PMID: 25043379). PARP11 has 4 isoforms with the molecular mass of 28-40 kDa.

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