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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PARP11 Polyclonal antibody (16692-1-AP)

    WB analysis using 16692-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16692-1-AP (PARP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PARP11 Polyclonal antibody (16692-1-AP)

    WB analysis of HeLa using 16692-1-AP

    WB result of PARP11 antibody (16692-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PARP11 transfected HeLa cells.

  • Western Blot (WB) analysis of PC-3 cells using PARP11 Polyclonal antibody (16692-1-AP)

    WB analysis of PC-3 using 16692-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16692-1-AP (PARP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PARP11 Polyclonal antibody (16692-1-AP)

    IHC staining of human kidney using 16692-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PARP11 Polyclonal antibody (16692-1-AP)

    IHC staining of human kidney using 16692-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PARP11 Polyclonal antibody (16692-1-AP)

    IHC staining of human placenta using 16692-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PARP11 Polyclonal antibody (16692-1-AP)

    IHC staining of human placenta using 16692-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16692-1-AP (PARP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using PARP11 Polyclonal antibody (16692-1-AP)

    IHC staining of human colon using 16692-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 16692-1-AP (PARP11 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PARP11 Polyclonal antibody (16692-1-AP)

    IHC staining of human liver using 16692-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PARP11 Polyclonal antibody (16692-1-AP)

    IHC staining of human liver using 16692-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PARP11 Polyclonal antibody (16692-1-AP)

    IHC staining of human ovary using 16692-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PARP11 Polyclonal antibody (16692-1-AP)

    IHC staining of human ovary using 16692-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PARP11 Polyclonal antibody (16692-1-AP)

    IHC staining of human testis using 16692-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PARP11 Polyclonal antibody (16692-1-AP)

    IHC staining of human testis using 16692-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using PARP11 Polyclonal antibody (16692-1-AP)

    IHC staining of human skin using 16692-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using PARP11 Polyclonal antibody (16692-1-AP)

    IHC staining of human skin using 16692-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16692-1-AP (PARP11 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using PARP11 Polyclonal antibody (16692-1-AP)

    IHC staining of human colon using 16692-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 16692-1-AP (PARP11 antibody) at dilution of 1:50 (under 40x lens).

  • Featured Product
  • KD/KO Validated

PARP11 Polyclonal antibody

PARP11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16692-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

ADP-ribosyltransferase diphtheria toxin-like 11, ARTD11, C12orf6, EC:2.4.2.-, MIB006

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, PC-3 cells, mouse heart tissue, mouse spleen tissue
Positive IHC detected inhuman kidney tissue, human placenta tissue, human colon tissue, human testis tissue, human skin tissue, human liver tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16692-1-AP targets PARP11 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10053

Product name: Recombinant human PARP11 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-331 aa of BC017569

Sequence: MFHKAEELFSKTTNNEVDDMDTSDTQWGWFYLAECGKWHMFQPDTNSQCSVSSEDIEKSFKTNPCGSISFTTSKFSYKIDFAEMKQMNLTTGKQRLIKRAPFSISAFSYICENEAIPMPPHWENVNTQVPYQLIPLHNQTHEYNEVANLFGKTMDRNRIKRIQRIQNLDLWEFFCRKKAQLKKKRGVPQINEQMLFHGTSSEFVEAICIHNFDWRINGIHGAVFGKGTYFARDAAYSSRFCKDDIKHGNTFQIHGVSLQQRHLFRTYKSMFLARVLIGDYINGDSKYMRPPSKDGSYVNLYDSCVDDTWNPKIFVVFDANQIYPEYLIDFH

相同性解析による交差性が予測される生物種
Full Name poly (ADP-ribose) polymerase family, member 11
Calculated molecular weight 331 aa, 39 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC017569
Gene Symbol PARP11
Gene ID (NCBI) 57097
RRIDAB_2158504
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR21
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PARP11, also named as ARTD11, belongs to the  ARTD/PARP family. PARP11 is a mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins. PARP11 plays a role in nuclear envelope stability and nuclear remodeling during spermiogenesis (PMID: 25043379). PARP11 has 4 isoforms with the molecular mass of 28-40 kDa.

Protocols

Product Specific Protocols
WB protocol for PARP11 antibody 16692-1-APDownload protocol
IHC protocol for PARP11 antibody 16692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pig,humanWB,IF

Front Cell Infect Microbiol

Identification of porcine PARP11 as a restricted factor for pseudorabies virus

Authors - Chunyun Qi
  • KD Validated
View Article