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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PARN Recombinant antibody (85408-5-RR)

    WB analysis using 85408-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85408-5-RR (PARN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of Human Breast Cancer(Her2+;ER-;PR-) using PARN Recombinant monoclonal antibody (85408-5-RR)

    IHC staining of Human Breast Cancer(Her2+;ER-;PR-) using 85408-5-RR

    Immunohistochemical analysis of paraffin-embedded Human Breast Cancer(Her2+;ER-;PR-) slide using 85408-5-RR (PARN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of Human Breast Cancer(Her2+;ER-;PR-) using PARN Recombinant monoclonal antibody (85408-5-RR)

    IHC staining of Human Breast Cancer(Her2+;ER-;PR-) using 85408-5-RR

    Immunohistochemical analysis of paraffin-embedded Human Breast Cancer(Her2+;ER-;PR-) slide using 85408-5-RR (PARN antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of A431 cells using PARN Recombinant monoclonal antibody (85408-5-RR)

    FC experiment of A431 using 85408-5-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug PARN Recombinant monoclonal antibody (85408-5-RR, Clone:242993F5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 85408-5-RR

    Affinity and Kinetic Characterization of 85408-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85408-5-RR against Human PARN were performed. The affinity constant is 96.9 pM.

PARN Recombinant monoclonal antibody

PARN Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 85408-5-RR
Clone No.242993F5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

242993F5, DAN, EC:3.1.13.4, Poly(A)-specific ribonuclease PARN, Polyadenylate-specific ribonuclease

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, MDA-MB-453 cells, HeLa cells, HEK-293T cells, HepG2 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inHuman Breast Cancer(Her2+;ER-;PR-)
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85408-5-RR targets PARN in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4774

Product name: Recombinant human PARN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-347 aa of BC050029

Sequence: MEIIRSNFKSNLHKVYQAIEEADFFAIDGEFSGISDGPSVSALTNGFDTPEERYQKLKKHSMDFLLFQFGLCTFKYDYTDSKYITKSFNFYVFPKPFNRSSPDVKFVCQSSSIDFLASQGFDFNKVFRNGIPYLNQEEERQLREQYDEKRSQANGAGALSYVSPNTSKCPVTIPEDQKKFIDQVVEKIEDLLQSEENKNLDLEPCTGFQRKLIYQTLSWKYPKGIHVETLETEKKERYIVISKVDEEERKRREQQKHAKEQEELNDAVGFSRVIHAIANSGKLVIGHNMLLDVMHTVHQFYCPLPADLSEFKEMTTCVFPRLLDTKLMASTQPFKDIINNTSLAELE

相同性解析による交差性が予測される生物種
Full Name poly(A)-specific ribonuclease (deadenylation nuclease)
Calculated molecular weight 639 aa, 73 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC050029
Gene Symbol PARN
Gene ID (NCBI) 5073
RRIDAB_3744201
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO95453
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Poly(A)-specific ribonuclease (PARN) is a deadenylase enzyme that is present in mammalian cells. As a deadenylase, PARN interacts with the cap and the poly(A) tail of mRNA to control the length of the poly(A) tail and regulate gene expression. Therefore, it plays a role in mRNA degradation in the nucleus and cytoplasm.

Protocols

Product Specific Protocols
FC protocol for PARN antibody 85408-5-RRDownload protocol
IHC protocol for PARN antibody 85408-5-RRDownload protocol
WB protocol for PARN antibody 85408-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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