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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PARN Recombinant monoclonal antibody (85408-5-RR)

    WB analysis using 85408-5-RR (same clone as 85408-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85408-5-RR (PARN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85408-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human Breast Cancer(Her2+;ER-;PR-) using PARN Recombinant monoclonal antibody (85408-5-RR)

    IHC staining of Human Breast Cancer(Her2+;ER-;PR-) using 85408-5-RR (same clone as 85408-5-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Breast Cancer(Her2+;ER-;PR-) slide using 85408-5-RR (PARN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85408-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human Breast Cancer(Her2+;ER-;PR-) using PARN Recombinant monoclonal antibody (85408-5-RR)

    IHC staining of Human Breast Cancer(Her2+;ER-;PR-) using 85408-5-RR (same clone as 85408-5-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Breast Cancer(Her2+;ER-;PR-) slide using 85408-5-RR (PARN antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85408-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85408-5-RR

    Affinity and Kinetic Characterization of 85408-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85408-5-RR against Human PARN were performed. The affinity constant is 96.9 pM.

PARN Recombinant monoclonal antibody, PBS Only

PARN Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85408-5-PBS
Clone No.242993F5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

242993F5, DAN, EC:3.1.13.4, Poly(A)-specific ribonuclease PARN, Polyadenylate-specific ribonuclease

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85408-5-PBS targets PARN in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4774

Product name: Recombinant human PARN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-347 aa of BC050029

Sequence: MEIIRSNFKSNLHKVYQAIEEADFFAIDGEFSGISDGPSVSALTNGFDTPEERYQKLKKHSMDFLLFQFGLCTFKYDYTDSKYITKSFNFYVFPKPFNRSSPDVKFVCQSSSIDFLASQGFDFNKVFRNGIPYLNQEEERQLREQYDEKRSQANGAGALSYVSPNTSKCPVTIPEDQKKFIDQVVEKIEDLLQSEENKNLDLEPCTGFQRKLIYQTLSWKYPKGIHVETLETEKKERYIVISKVDEEERKRREQQKHAKEQEELNDAVGFSRVIHAIANSGKLVIGHNMLLDVMHTVHQFYCPLPADLSEFKEMTTCVFPRLLDTKLMASTQPFKDIINNTSLAELE

相同性解析による交差性が予測される生物種
Full Name poly(A)-specific ribonuclease (deadenylation nuclease)
Calculated molecular weight 639 aa, 73 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC050029
Gene Symbol PARN
Gene ID (NCBI) 5073
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO95453
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Poly(A)-specific ribonuclease (PARN) is a deadenylase enzyme that is present in mammalian cells. As a deadenylase, PARN interacts with the cap and the poly(A) tail of mRNA to control the length of the poly(A) tail and regulate gene expression. Therefore, it plays a role in mRNA degradation in the nucleus and cytoplasm.

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