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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PAPSS2 Polyclonal antibody (15978-1-AP)

    WB analysis of HepG2 using 15978-1-AP (same clone as 15978-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15978-1-AP (PAPSS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15978-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using PAPSS2 Polyclonal antibody (15978-1-AP)

    WB analysis of human liver using 15978-1-AP (same clone as 15978-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 15978-1-AP (PAPSS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15978-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PAPSS2 Polyclonal antibody (15978-1-AP)

    IHC staining of mouse liver using 15978-1-AP (same clone as 15978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15978-1-AP (PAPSS2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15978-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PAPSS2 Polyclonal antibody (15978-1-AP)

    IHC staining of mouse liver using 15978-1-AP (same clone as 15978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15978-1-AP (PAPSS2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15978-1-PBS in a different storage buffer formulation.

PAPSS2 Polyclonal antibody, PBS Only

PAPSS2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15978-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

3'-phosphoadenosine-5'-phosphosulfate synthase, Adenosine-5'-phosphosulfate 3'-phosphotransferase, Adenylylsulfate 3'-phosphotransferase, Adenylyl-sulfate kinase, ATPSK2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15978-1-PBS targets PAPSS2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8766

Product name: Recombinant human PAPSS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-333 aa of BC009894

Sequence: MSGIKKQKTENQQKSTNVVYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTISFALEEYLVSHAIPCYSLDGDNVRHGLNRNLGFSPGDREENIRRIAEVAKLFADAGLVCITSFISPFAKDRENARKIHESAGLPFFEIFVDAPLNICESRDVKGLYKRARAGEIKGFTGIDSDYEKPETPERVLKTNLSTVSDCVHQVVELLQEQNIVPYTIIKDIHELFVPENKLDHVRAEAETLPSLSITKLDLQWVQVLSEGWATPLKGFMREKEYLQVMHFDTLLDDGVINMSIPIVLPVSAEDKTRLEGCSKFVLAHGGRRVAILRDAEF

相同性解析による交差性が予測される生物種
Full Name 3'-phosphoadenosine 5'-phosphosulfate synthase 2
Calculated molecular weight 614 aa, 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC009894
Gene Symbol PAPSS2
Gene ID (NCBI) 9060
RRIDAB_2159498
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95340
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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