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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PAPSS2 Polyclonal antibody (15978-1-AP)

    WB analysis using 15978-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15978-1-AP (PAPSS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PAPSS2 Polyclonal antibody (15978-1-AP)

    IHC staining of mouse liver using 15978-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15978-1-AP (PAPSS2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PAPSS2 Polyclonal antibody (15978-1-AP)

    IHC staining of mouse liver using 15978-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15978-1-AP (PAPSS2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PAPSS2 Polyclonal antibody

PAPSS2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15978-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

3'-phosphoadenosine-5'-phosphosulfate synthase, Adenosine-5'-phosphosulfate 3'-phosphotransferase, Adenylylsulfate 3'-phosphotransferase, Adenylyl-sulfate kinase, ATPSK2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells, HT-29 cells, NCI-H1299 cells, RKO cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15978-1-AP targets PAPSS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8766

Product name: Recombinant human PAPSS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-333 aa of BC009894

Sequence: MSGIKKQKTENQQKSTNVVYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTISFALEEYLVSHAIPCYSLDGDNVRHGLNRNLGFSPGDREENIRRIAEVAKLFADAGLVCITSFISPFAKDRENARKIHESAGLPFFEIFVDAPLNICESRDVKGLYKRARAGEIKGFTGIDSDYEKPETPERVLKTNLSTVSDCVHQVVELLQEQNIVPYTIIKDIHELFVPENKLDHVRAEAETLPSLSITKLDLQWVQVLSEGWATPLKGFMREKEYLQVMHFDTLLDDGVINMSIPIVLPVSAEDKTRLEGCSKFVLAHGGRRVAILRDAEF

相同性解析による交差性が予測される生物種
Full Name 3'-phosphoadenosine 5'-phosphosulfate synthase 2
Calculated molecular weight 614 aa, 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC009894
Gene Symbol PAPSS2
Gene ID (NCBI) 9060
RRIDAB_2159498
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95340
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for PAPSS2 antibody 15978-1-APDownload protocol
WB protocol for PAPSS2 antibody 15978-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Cell Rep Med

Neuroprotective response against the onset of ischemic stroke by upregulation of histone H3Y99 sulfation.

Authors - Li Jiang
View Article

Discov Oncol

Application of Mendelian randomization and bioinformatic analysis to construct a prognostic model for thyroid cancer and perform pan-cancer analysis

Authors - Zhenrun Zhan
View Article

Hum Reprod

PAPSS2-mediated BMPR1A sulfation in stromal cells regulating endometrial decidualization via autophagy.

Authors - Duo Zi
View Article
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