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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PACSIN2 Recombinant antibody (84763-4-RR)

    WB analysis using 84763-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84763-4-RR (PACSIN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PACSIN2 Recombinant antibody (84763-4-RR)

    IHC staining of human breast cancer using 84763-4-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 84763-4-RR (PACSIN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with .

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using PACSIN2 Recombinant antibody (84763-4-RR)

    IF Staining of NIH/3T3 using 84763-4-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PACSIN2 antibody (84763-4-RR, Clone: 242193H3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PACSIN2 Recombinant antibody (84763-4-RR)

    IF Staining of HepG2 using 84763-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PACSIN2 antibody (84763-4-RR, Clone: 242193H3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84763-4-RR

    Affinity and Kinetic Characterization of 84763-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84763-4-RR against Human PACSIN2 were performed. The affinity constant is 3.72 nM.

PACSIN2 Recombinant monoclonal antibody

PACSIN2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84763-4-RR
Clone No.242193H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

242193H3, Protein kinase C and casein kinase substrate in neurons protein 2, SdpII, Syndapin-2, Syndapin-II

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84763-4-RR targets PACSIN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0809

Product name: Recombinant human PACSIN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 180-486 aa of BC008037

Sequence: PSLNPEQLKKLQDKIEKCKQDVLKTKEKYEKSLKELDQGTPQYMENMEQVFEQCQQFEEKRLRFFREVLLEVQKHLDLSNVAGYKAIYHDLEQSIRAADAVEDLRWFRANHGPGMAMNWPQFEEWSADLNRTLSRREKKKATDGVTLTGINQTGDQSLPSKPSSTLNVPSNPAQSAQSQSSYNPFEDEDDTGSTVSEKDDTKAKNVSSYEKTQSYPTDWSDDESNNPFSSTDANGDSNPFDDDATSGTEVRVRALYDYEGQEHDELSFKAGDELTKMEDEDEQGWCKGRLDNGQVGLYPANYVEAIQ

相同性解析による交差性が予測される生物種
Full Name protein kinase C and casein kinase substrate in neurons 2
Calculated molecular weight 56 kDa
Observed molecular weight60 kDa
GenBank accession numberBC008037
Gene Symbol PACSIN2
Gene ID (NCBI) 11252
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9UNF0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peripheral membrane proteins of the Bin/amphiphysin/Rvs (BAR) and Fer-CIP4 homology-BAR (F-BAR) family participate in cellular membrane trafficking (PMID: 19549836). PACSIN2 (also known as syndapin-2) is a member of the protein kinase C and casein kinase substrate in neurons (PACSIN) family, which are membrane-binding proteins characterized by an amino-terminal F-BAR domain. PACSIN2 plays a role in intracellular vesicle-mediated transport. It is involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization (PMID: 23129763). PACSIN2 also has a role in caveolae membrane sculpting (PMID: 21610094).

Protocols

Product Specific Protocols
IF protocol for PACSIN2 antibody 84763-4-RRDownload protocol
IHC protocol for PACSIN2 antibody 84763-4-RRDownload protocol
WB protocol for PACSIN2 antibody 84763-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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