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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PACSIN2 Recombinant antibody (84763-4-RR)

    WB analysis using 84763-4-RR (same clone as 84763-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84763-4-RR (PACSIN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84763-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PACSIN2 Recombinant antibody (84763-4-RR)

    IHC staining of human breast cancer using 84763-4-RR (same clone as 84763-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 84763-4-RR (PACSIN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 84763-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using PACSIN2 Recombinant antibody (84763-4-RR)

    IF Staining of NIH/3T3 using 84763-4-RR (same clone as 84763-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PACSIN2 antibody (84763-4-RR, Clone: 242193H3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84763-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PACSIN2 Recombinant antibody (84763-4-RR)

    IF Staining of HepG2 using 84763-4-RR (same clone as 84763-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PACSIN2 antibody (84763-4-RR, Clone: 242193H3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84763-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84763-4-RR

    Affinity and Kinetic Characterization of 84763-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84763-4-RR against Human PACSIN2 were performed. The affinity constant is 3.72 nM.

PACSIN2 Recombinant monoclonal antibody, PBS Only

PACSIN2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84763-4-PBS
Clone No.242193H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

242193H3, Protein kinase C and casein kinase substrate in neurons protein 2, SdpII, Syndapin-2, Syndapin-II

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84763-4-PBS targets PACSIN2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0809

Product name: Recombinant human PACSIN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 180-486 aa of BC008037

Sequence: PSLNPEQLKKLQDKIEKCKQDVLKTKEKYEKSLKELDQGTPQYMENMEQVFEQCQQFEEKRLRFFREVLLEVQKHLDLSNVAGYKAIYHDLEQSIRAADAVEDLRWFRANHGPGMAMNWPQFEEWSADLNRTLSRREKKKATDGVTLTGINQTGDQSLPSKPSSTLNVPSNPAQSAQSQSSYNPFEDEDDTGSTVSEKDDTKAKNVSSYEKTQSYPTDWSDDESNNPFSSTDANGDSNPFDDDATSGTEVRVRALYDYEGQEHDELSFKAGDELTKMEDEDEQGWCKGRLDNGQVGLYPANYVEAIQ

相同性解析による交差性が予測される生物種
Full Name protein kinase C and casein kinase substrate in neurons 2
Calculated molecular weight 56 kDa
Observed molecular weight60 kDa
GenBank accession numberBC008037
Gene Symbol PACSIN2
Gene ID (NCBI) 11252
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9UNF0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Peripheral membrane proteins of the Bin/amphiphysin/Rvs (BAR) and Fer-CIP4 homology-BAR (F-BAR) family participate in cellular membrane trafficking (PMID: 19549836). PACSIN2 (also known as syndapin-2) is a member of the protein kinase C and casein kinase substrate in neurons (PACSIN) family, which are membrane-binding proteins characterized by an amino-terminal F-BAR domain. PACSIN2 plays a role in intracellular vesicle-mediated transport. It is involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization (PMID: 23129763). PACSIN2 also has a role in caveolae membrane sculpting (PMID: 21610094).

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