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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated P62,SQSTM1 Monoclonal antibody

P62,SQSTM1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H5C1

Cat no : CL594-66184

Synonyms

A170, EBIAP, ORCA, OSIL, p60, P62, P62/SQSTM1, p62B, PDB3, sequestosome 1, SQSTM1, Ubiquitin binding protein p62, ZIP3

Validation Data Gallery

Applications:
  • IF Staining of U2OS using CL594-66184

    IF Staining of U2OS using CL594-66184

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated U2OS cells using CoraLite®594 P62/SQSTM1 antibody (CL594-66184, Clone: 1H5C1 ) at dilution of 1:100, CL488-Phalloidin (green).

  • IF Staining of HepG2 using CL594-66184

    IF Staining of HepG2 using CL594-66184

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using CoraLite®594-conjugated P62/SQSTM1 antibody (CL594-66184, Clone: 1H5C1 ) at dilution of 1:100.

  • IF Staining of HepG2 using CL594-66184

    IF Staining of HepG2 using CL594-66184

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using CL594-66184 (P62,SQSTM1 antibody) at dilution of 1:100. .

Tested Applications

Positive IF detected inHepG2 cells, Chloroquine treated U2OS cells
For other applications, we recommend the unconjugated version of this antibody, 66184-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66184 targets P62,SQSTM1 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen P62,SQSTM1 fusion protein Ag13131 相同性解析による交差性が予測される生物種
Full Name sequestosome 1
Calculated molecular weight 48 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC017222
Gene symbol SQSTM1
Gene ID (NCBI) 8878
RRIDAB_2883528
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (PMID: 22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.

Protocols

Product Specific Protocols
IF protocol for CL594 P62,SQSTM1 antibody CL594-66184Download protocol
Standard Protocols
Click here to view our Standard Protocols