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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of mouse pituitary gland tissue using OXT Polyclonal antibody (18041-1-AP)

    IP experiment of mouse pituitary gland using 18041-1-AP (same clone as 18041-1-PBS)

    IP result of anti-OXT (IP:18041-1-AP, 4ug; Detection:18041-1-AP 1:300) with mouse pituitary gland tissue lysate 1440 ug. This data was developed using the same antibody clone with 18041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pituitary tissue using OXT Polyclonal antibody (18041-1-AP)

    IHC staining of human pituitary using 18041-1-AP (same clone as 18041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pituitary tissue using OXT Polyclonal antibody (18041-1-AP)

    IHC staining of human pituitary using 18041-1-AP (same clone as 18041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using OXT Polyclonal antibody (18041-1-AP)

    IHC staining of mouse brain using 18041-1-AP (same clone as 18041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using OXT Polyclonal antibody (18041-1-AP)

    IHC staining of mouse ovary using 18041-1-AP (same clone as 18041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using OXT Polyclonal antibody (18041-1-AP)

    IHC staining of mouse ovary using 18041-1-AP (same clone as 18041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18041-1-PBS in a different storage buffer formulation.

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OXT Polyclonal antibody, PBS Only

OXT Polyclonal Antibody for IHC, IP, Indirect ELISA
Cat No. 18041-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, Indirect ELISA

Synonyms

Neurophysin 1, Ocytocin Neurophysin 1, Oxytocin, Oxytocin neurophysin 1, Oxytocin-neurophysin 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18041-1-PBS targets OXT in IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OXT fusion protein Ag12672 相同性解析による交差性が予測される生物種
Full Name oxytocin, prepropeptide
Calculated molecular weight 125 aa, 13 kDa
Observed molecular weight13 kDa
GenBank accession numberBC101841
Gene Symbol OXT
Gene ID (NCBI) 5020
RRIDAB_2918056
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01178
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Oxytocin (OXT) is a neuropeptide involved in social-approach behaviors in humans and others mammals (PMID: 27325757). OXT is located on chromosome 20p13 and codes for a precursor protein that is synthetized to produce oxytocin and neurophysin I, and neurophysin I is the carrier protein for oxytocin (PMID: 1486803, PMID: 18566739). Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. Acts by binding to oxytocin receptor (OXTR) (PMID: 18174156).