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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of mouse pituitary gland tissue using Oxytocin-neurophysin 1 Polyclonal antibody (18041-1-AP)

    IP experiment of mouse pituitary gland using 18041-1-AP (same clone as 18041-1-PBS)

    IP result of anti-OXT (IP:18041-1-AP, 4ug; Detection:18041-1-AP 1:300) with mouse pituitary gland tissue lysate 1440 ug. This data was developed using the same antibody clone with 18041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pituitary tissue using Oxytocin-neurophysin 1 Polyclonal antibody (18041-1-AP)

    IHC staining of human pituitary using 18041-1-AP (same clone as 18041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pituitary tissue using Oxytocin-neurophysin 1 Polyclonal antibody (18041-1-AP)

    IHC staining of human pituitary using 18041-1-AP (same clone as 18041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Oxytocin-neurophysin 1 Polyclonal antibody (18041-1-AP)

    IHC staining of mouse brain using 18041-1-AP (same clone as 18041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using Oxytocin-neurophysin 1 Polyclonal antibody (18041-1-AP)

    IHC staining of mouse ovary using 18041-1-AP (same clone as 18041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using Oxytocin-neurophysin 1 Polyclonal antibody (18041-1-AP)

    IHC staining of mouse ovary using 18041-1-AP (same clone as 18041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18041-1-PBS in a different storage buffer formulation.

Oxytocin-neurophysin 1 Polyclonal antibody, PBS Only

Oxytocin-neurophysin 1 Polyclonal Antibody for IHC, IP, Indirect ELISA
Cat No. 18041-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, Indirect ELISA

Synonyms

OXT, Neurophysin 1, Ocytocin Neurophysin 1, Oxytocin, Oxytocin neurophysin 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18041-1-PBS targets Oxytocin-neurophysin 1 in IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12672

Product name: Recombinant human OXT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 19-125 aa of BC101841

Sequence: ACYIQNCPLGGKRAAPDLDVRKCLPCGPGGKGRCFGPNICCAEELGCFVGTAEALRCQEENYLPSPCQSGQKACGSGGRCAVLGLCCSPDGCHADPACDAEATFSQR

相同性解析による交差性が予測される生物種
Full Name oxytocin, prepropeptide
Calculated molecular weight 125 aa, 13 kDa
Observed molecular weight13 kDa
GenBank accession numberBC101841
Gene Symbol OXT
Gene ID (NCBI) 5020
RRIDAB_2918056
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP01178
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Oxytocin (OXT) is a neuropeptide involved in social-approach behaviors in humans and others mammals (PMID: 27325757). OXT is located on chromosome 20p13 and codes for a precursor protein that is synthetized to produce oxytocin and neurophysin I, and neurophysin I is the carrier protein for oxytocin (PMID: 1486803, PMID: 18566739). Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. Acts by binding to oxytocin receptor (OXTR) (PMID: 18174156).

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