Validation Data Gallery
Tested Applications
| Positive IP detected in | mouse pituitary gland tissue |
| Positive IHC detected in | human pituitary tissue, mouse ovary tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
18041-1-AP targets Oxytocin-neurophysin 1 in IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12672 Product name: Recombinant human OXT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 19-125 aa of BC101841 Sequence: ACYIQNCPLGGKRAAPDLDVRKCLPCGPGGKGRCFGPNICCAEELGCFVGTAEALRCQEENYLPSPCQSGQKACGSGGRCAVLGLCCSPDGCHADPACDAEATFSQR 相同性解析による交差性が予測される生物種 |
| Full Name | oxytocin, prepropeptide |
| Calculated molecular weight | 125 aa, 13 kDa |
| Observed molecular weight | 13 kDa |
| GenBank accession number | BC101841 |
| Gene Symbol | OXT |
| Gene ID (NCBI) | 5020 |
| RRID | AB_2918056 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01178 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Oxytocin-neurophysin 1, also called the oxytocin/neurophysin I prepropeptide, is produced in the hypothalamus as a single, inactive precursor that contains two parts: the nonapeptide hormone oxytocin and its carrier protein neurophysin I. After synthesis in magnocellular neurons of the supra-optic and paraventricular nuclei, the precursor is packaged into neurosecretory vesicles and transported down the axon to the posterior pituitary (neurohypophysis). During this axonal transport the precursor is proteolytically cleaved, generating mature oxytocin and its tightly bound neurophysin I; the complex is stored in nerve terminals and secreted into the systemic circulation upon appropriate stimulation.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Oxytocin-neurophysin 1 antibody 18041-1-AP | Download protocol |
| IP protocol for Oxytocin-neurophysin 1 antibody 18041-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |