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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ORM1 Polyclonal antibody (16439-1-AP)

    WB analysis using 16439-1-AP (same clone as 16439-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16439-1-AP (ORM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse bladder tissue using ORM1 Polyclonal antibody (16439-1-AP)

    WB analysis of mouse bladder using 16439-1-AP (same clone as 16439-1-PBS)

    mouse bladder tissue were subjected to SDS PAGE followed by western blot with 16439-1-AP (ORM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of human plasma using ORM1 Polyclonal antibody (16439-1-AP)

    IP experiment of human plasma using 16439-1-AP (same clone as 16439-1-PBS)

    IP result of anti-ORM1 (IP:16439-1-AP, 3ug; Detection:16439-1-AP 1:300) with human plasma lysate 100ug. This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ORM1 Polyclonal antibody (16439-1-AP)

    IHC staining of human liver using 16439-1-AP (same clone as 16439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16439-1-AP (ORM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ORM1 Polyclonal antibody (16439-1-AP)

    IHC staining of human liver using 16439-1-AP (same clone as 16439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16439-1-AP (ORM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ORM1 Polyclonal antibody (16439-1-AP)

    IHC staining of human colon using 16439-1-AP (same clone as 16439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16439-1-AP (ORM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ORM1 Polyclonal antibody (16439-1-AP)

    IHC staining of human liver cancer using 16439-1-AP (same clone as 16439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16439-1-AP (ORM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ORM1 Polyclonal antibody (16439-1-AP)

    IHC staining of human liver cancer using 16439-1-AP (same clone as 16439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16439-1-AP (ORM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ORM1 Polyclonal antibody (16439-1-AP)

    IF Staining of HepG2 using 16439-1-AP (same clone as 16439-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ORM1 antibody (16439-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using ORM1 Polyclonal antibody (16439-1-AP)

    FC experiment of HepG2 using 16439-1-AP (same clone as 16439-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ORM1 Polyclonal antibody (16439-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 16439-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ORM1 Polyclonal antibody, PBS Only

ORM1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 16439-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16439-1-PBS targets ORM1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9758

Product name: Recombinant human ORM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 25-201 aa of BC026238

Sequence: NLVPVPITNATLDRITGKWFYIASAFRNEEYNKSVQEIQATFFYFTPNKTEDTIFLREYQTRQDQCIYNTTYLNVQRENGTISRYVGGQEHFAHLLILRDTKTYMLAFDVNDEKNWGLSVYADKPETTKEQLGEFYEALDCLRIPKSDVVYTDWKKDKCEPLEKQHEKERKQEEGES

相同性解析による交差性が予測される生物種
Full Name orosomucoid 1
Calculated molecular weight 201 aa, 24 kDa
Observed molecular weight 40-47 kDa
GenBank accession numberBC026238
Gene Symbol ORM1
Gene ID (NCBI) 5004
RRIDAB_2878259
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP02763
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Alpha-1-acid glycoprotein 1 (AGP1), also called orosomucoid-1 (ORM1), is a glycoprotein synthesized mostly by hepatocytes and present in human plasma. ORM1 is an acute-phase reactant protein controlled by glucocorticoids, interleukin-1 and interleukin-6, and increase up to 5-50 times upon infection and/or inflammation. Anti-apoptotic effect and role as immunomodulator of ORM have been reported. ORM is an important carrier for synthetic drugs and influences their distribution and availability in the body. This antibody recognizes a band about 44 kDa in human plasma which may be due to the glycosylation of ORM1 or the dimer formation of the protein.

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