Validation Data Gallery
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Tested Applications
| Positive WB detected in | COLO 320 cells, A549 cells, L02 cells, HEK-293 cells |
| Positive IP detected in | COLO 320 cells |
| Positive IF/ICC detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85998-1-RR targets OGFR in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | OGFR fusion protein Ag1671 相同性解析による交差性が予測される生物種 |
| Full Name | opioid growth factor receptor |
| Calculated molecular weight | 73 kDa |
| Observed molecular weight | 110 kDa |
| GenBank accession number | BC014137 |
| Gene Symbol | OGFR |
| Gene ID (NCBI) | 11054 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NZT2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OGFR antibody 85998-1-RR | Download protocol |
| IF protocol for OGFR antibody 85998-1-RR | Download protocol |
| IP protocol for OGFR antibody 85998-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |