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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OGFR Recombinant antibody (85998-1-RR)

    WB analysis using 85998-1-RR (same clone as 85998-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85998-1-RR (OGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85998-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using OGFR Recombinant antibody (85998-1-RR)

    IP experiment of COLO 320 using 85998-1-RR (same clone as 85998-1-PBS)

    IP result of anti-OGFR (IP:85998-1-RR, 4ug; Detection:85998-1-RR 1:3000) with COLO 320 cells lysate 1920 ug. This data was developed using the same antibody clone with 85998-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using OGFR Recombinant antibody (85998-1-RR)

    IF Staining of L02 using 85998-1-RR (same clone as 85998-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using OGFR antibody (85998-1-RR, Clone: 250452E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85998-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85998-1-RR

    Affinity and Kinetic Characterization of 85998-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85998-1-RR against Human OGFR were performed. The affinity constant is 0.211 nM.

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OGFR Recombinant antibody, PBS Only

OGFR Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 85998-1-PBS
Clone No.250452E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Opioid growth factor receptor, Protein 7 60, Protein 7-60, Zeta type opioid receptor, Zeta-type opioid receptor

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85998-1-PBS targets OGFR in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen OGFR fusion protein Ag1671 相同性解析による交差性が予測される生物種
Full Name opioid growth factor receptor
Calculated molecular weight 73 kDa
Observed molecular weight110 kDa
GenBank accession numberBC014137
Gene Symbol OGFR
Gene ID (NCBI) 11054
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NZT2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.