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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Nicastrin Recombinant monoclonal antibody (87471-1-RR)

    WB analysis using 87471-1-RR (same clone as 87471-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87471-1-RR (Nicastrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87471-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Nicastrin Recombinant monoclonal antibody (87471-1-RR)

    WB analysis using 87471-1-RR (same clone as 87471-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87471-1-RR (Nicastrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87471-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using Nicastrin Recombinant monoclonal antibody (87471-1-RR)

    IF Staining of C6 using 87471-1-RR (same clone as 87471-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C6 cells using Nicastrin antibody (87471-1-RR, Clone: 252886F8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87471-1-PBS in a different storage buffer formulation.

  • Affinity of 87471-1-RR

    Affinity of 87471-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87471-RR against Human Nicastrin were performed. The affinity constant is 1.23 nM.

Nicastrin Recombinant monoclonal antibody, PBS Only

Nicastrin Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87471-1-PBS
Clone No.252886F8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

KIAA0253, NCSTN, UNQ1874/PRO4317

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87471-1-PBS targets Nicastrin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag5137

Product name: Recombinant human Nicastrin protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 338-689 aa of BC047621

Sequence: NVDSFVELGQVALRTSLELWMHTDPVSQKNESVRNQVEDLLATLEKSGAGVPAVILRRPNQSQPLPPSSLQRFLRARNISGVVLADHSGAFHNKYYQSIYDTAENINVSYPEWLSPEEDLNFVTDTAKALADVATVLGRALYELAGGTNFSDTVQADPQTVTRLLYGFLIKANNSWFQSILRQDLRSYLGDGPLQHYIAVSSPTNTTYVVQYALANLTGTVVNLTREQCQDPSKVPSENKDLYEYSWVQGPLHSNETDRLPRCVRSTARLARALSPAFELSQWSSTEYSTWTESRWKDIHARIFLIASKELELITLTVGFGILIFSLIVTYCINAKADVLFIAPREPGAVSY

相同性解析による交差性が予測される生物種
Full Name nicastrin
Calculated molecular weight 709 aa, 78.4 kDa
Observed molecular weight 110 kDa, 130 kDa
GenBank accession numberBC047621
Gene Symbol Nicastrin
Gene ID (NCBI) 23385
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ92542
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Nicastrin (NCT) is a type I transmembrane glycoprotein, which forms high molecular weight gamma-secretase complexes with presenilin 1 and presenilin 2 (PMID: 16096051). The gamma-secretase is an intramembrane protease that cleaves many type I membrane proteins, including amyloid precursor protein (APP) and Notch, generating peptide fragments that are important signaling components (PMID: 16096062). The proteolytic processing of APP results in the release of amyloid-βpeptide, a principal component of the plaques found in the brains of Alzheimer's disease patients (PMID: 12716934).

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