Validation Data Gallery
Tested Applications
| Positive FC detected in | HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
Biotin-FcA98210 targets Neuropilin 1/CD304 in FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg2331 Product name: Recombinant Human Neuropilin-1 protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 22-644 aa of BC007533 Sequence: FRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFVPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTNWSAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWITIKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRIKPATWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPAPHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSGIK 相同性解析による交差性が予測される生物種 |
| Full Name | neuropilin 1 |
| GenBank accession number | BC007533 |
| Gene Symbol | Neuropilin 1 |
| Gene ID (NCBI) | 8829 |
| Conjugate | Biotin |
| Excitation/Emission maxima wavelengths | - |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O14786 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Neuropilin 1 (NRP1), also known as CD304 or VEGF165R, is a type I transmembrane proteins consisting of a small intracellular cytoplasmic domain, a transmembrane domain and an extracellular domain containing a complement-binding CUB domain (a1/a2), a coagulation factor V/VIII (b1/b2) domain, and a meprin or MAM domain (PMID: 24263240; 11886873). It is expressed on plasmacytoid dendritic cells (pDCs), endothelial cells, thymocytes, regulatory T cells, a subset of T follicular helper cells, neurons, and diverse human solid tumors (PMID: 31944572; 14991591; 18704117). Neuropilin 1 is involved in immunity, cardiovascular development, neuronal guidance, cell migration, angiogenesis and cancer pathogenesis (PMID: 24263240).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Biotin Neuropilin 1/CD304 antibody Biotin-FcA98210 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |