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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Nestin Polyclonal antibody (29285-1-AP)

    WB analysis using 29285-1-AP (same clone as 29285-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29285-1-AP (nestin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29285-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Nestin Polyclonal antibody (29285-1-AP)

    WB analysis using 29285-1-AP (same clone as 29285-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 29285-1-AP (nestin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29285-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Nestin Polyclonal antibody (29285-1-AP)

    IF Staining of rat brain using 29285-1-AP (same clone as 29285-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using nestin antibody (29285-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), NeuN antibody (66836-1-Ig, Clone: 3A4C1, red). This data was developed using the same antibody clone with 29285-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using Nestin Polyclonal antibody (29285-1-AP)

    IF Staining of human kidney using 29285-1-AP (same clone as 29285-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Nestin antibody (29285-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29285-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Nestin Polyclonal antibody (29285-1-AP)

    IF Staining of rat brain using 29285-1-AP (same clone as 29285-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using nestin antibody (29285-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), GFAP antibody (60190-1-Ig, Clone: 4B2E10, red). This data was developed using the same antibody clone with 29285-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using Nestin Polyclonal antibody (29285-1-AP)

    IF Staining of U-251 using 29285-1-AP (same clone as 29285-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using Nestin antibody (29285-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29285-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using Nestin Polyclonal antibody (29285-1-AP)

    FC experiment of SH-SY5Y using 29285-1-AP (same clone as 29285-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Nestin (29285-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 29285-1-PBS in a different storage buffer formulation.

Nestin Polyclonal antibody, PBS Only

Nestin Polyclonal Antibody for WB, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 29285-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Synonyms

NES

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29285-1-PBS targets Nestin in WB, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag29865

Product name: Recombinant human nestin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1450-1621 aa of NM_006617

Sequence: SSSQRGEFLESDSVSVSVPWDDSLRGAVAGAPKTALETESQDSAEPSGSEEESDPVSLEREDKVPGPLEIPSGMEDAGPGADIIGVNGQGPNLEGKSQHVNGGVMNGLEQSEEVGQGMPLVSEGDRGSPFQEEEGSALKTSWAGAPVHLGQGQFLKFTQREGDRESWSSGED

相同性解析による交差性が予測される生物種
Full Name nestin
Calculated molecular weight 177 kDa
Observed molecular weight240-280 kDa
GenBank accession numberNM_006617
Gene Symbol Nestin
Gene ID (NCBI) 10763
RRIDAB_2918268
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP48681
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).

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