Nestin Polyclonal antibody
Nestin Polyclonal Antibody for WB, IF, FC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, rat, mouse and More (1)
Applications
WB, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 29285-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | SGC-7901 cells, U-251 cells, U-87 MG cells |
Positive IF detected in | rat brain tissue, mouse kidney tissue, human kidney tissue, U-251 cells |
Positive FC detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Published Applications
IF | See 1 publications below |
Product Information
29285-1-AP targets Nestin in WB, IF, FC, ELISA applications and shows reactivity with Human, rat, mouse samples.
Tested Reactivity | Human, rat, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Nestin fusion protein Ag29865 相同性解析による交差性が予測される生物種 |
Full Name | nestin |
Calculated molecular weight | 177 kDa |
Observed molecular weight | 240-280 kDa |
GenBank accession number | NM_006617 |
Gene symbol | NES |
Gene ID (NCBI) | 10763 |
RRID | AB_2918268 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).
Protocols
Product Specific Protocols | |
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WB protocol for Nestin antibody 29285-1-AP | Download protocol |
IF protocol for Nestin antibody 29285-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Bioact Mater Construction of functional neural network tissue combining CBD-NT3-modified linear-ordered collagen scaffold and TrkC-modified iPSC-derived neural stem cells for spinal cord injury repair |