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Validation Data Gallery

  • Immunofluorescence analysis of HeLa cells stained with mouse IgG2b anti-Tubulin beta antibody and Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, CoraLite® Plus 555 (smsG2bCL555-1, orange). Nuclei were stained with DAPI (blue). Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Immunofluorescence analysis of HeLa cells stained with mouse IgG2b anti-Tubulin beta antibody and Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, CoraLite® Plus 555 (smsG2bCL555-1, orange). Nuclei were stained with DAPI (blue). Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • HEK-293 cell lysates were subjected to SDS-PAGE followed by multiplex western blot analysis with 3 mouse primary antibodies including anti-ATP50 (66696-1-Ig), anti-AIF (67791-1-Ig), and anti-actin (66009-1-Ig). Primary antibodies were detected using 3 mouse IgG subclass-specific nano-secondary reagents including Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, CoraLite® Plus 750 (smsG1CL750-1, white), Nano-Secondary® alpaca anti-mouse IgG2a, recombinant VHH, CoraLite® Plus 488 (smsG2aCL488-1, green), and Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, CoraLite® Plus 555 (smsG2bCL555-1, red).

    HEK-293 cell lysates were subjected to SDS-PAGE followed by multiplex western blot analysis with 3 mouse primary antibodies including anti-ATP50 (66696-1-Ig), anti-AIF (67791-1-Ig), and anti-actin (66009-1-Ig). Primary antibodies were detected using 3 mouse IgG subclass-specific nano-secondary reagents including Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, CoraLite® Plus 750 (smsG1CL750-1, white), Nano-Secondary® alpaca anti-mouse IgG2a, recombinant VHH, CoraLite® Plus 488 (smsG2aCL488-1, green), and Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, CoraLite® Plus 555 (smsG2bCL555-1, red).

ChromoTek Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, CoraLite® Plus 555 [CTK0105, CTK0106]

Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity.
Cat No. smsG2bCL555-1
Publications(1)

Applications

IF, WB

Reactivity

mouse IgG2b

Host

Alpaca

Conjugate

CoraLite® Plus 555

Synonyms

Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

Size/Concentration: 

-/ -

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Product Information

Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity.

DescriptionNano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below.
ApplicationsIF, WB
HostAlpaca-derived, recombinantly produced in bacteria
Species ReactivityMouse IgG2b Fc-fragment
No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG1, IgG2a, IgG2c, IgG3, IgM
Target/SpecificityFc-fragment of mouse IgG2b
Physical StateLiquid
ConjugateCoraLite® Plus 555
Suggested DilutionImmunofluorescence 1: 500 - 1:1,000
Western blot 1: 500 - 1:1,000
Purification MethodRecombinant expression, affinity purification IMAC
TypeSecondary Nanobody
ClassRecombinant
FormatAlpaca single domain antibody, monovalent
Cross-reactivityNo cross-reactivity to goat, guinea pig, human, macaque, rabbit, rat, and sheep serum and to mouse IgG1, IgG2a, IgG2c, IgG3, and IgM
ImmunogenPurified mouse IgG2b
ClonalityBiclonal: mixture of 2 monoclonal Nanobodies
ClonesCTK0105 (VHH0275), CTK106 (VHH0288)
Affinity (KD)CTK0105: KD = 5 nM, CTK0106: KD = 0.2 nM
RRIDAB_2941315
Excitation / EmissionExcitation max: 554 nm, Emission max: 570 nm
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
Storage ConditionShipped at ambient temperature. Store at +4°C short term or -20°C long term. Stable for 1 year at -20°C.
Size10 μL; 100 μL
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

Medicina (Kaunas)

Immunological Landscape of Non-Melanoma Skin Neoplasms: Role of CTLA4+IFN-γ+ Lymphocytes in Tumor Microenvironment Suppression

Authors - Silvana Karabatić Knezović
View Article