Capture of Fab fragments (Biolayer Interferometry)
The Nano-CaptureLigand™ human Ig, kappa LC-specific VHH, biotinylated, was immobilized on Streptavidin biosensors and used to capture Fab fragments (κ light chain) of therapeutic human IgGs . Association for 600 s followed by dissociation for 600 s.

Kinetic analysis of the Adalimumab Fab fragment via Biolayer Interferometry
The Nano-CaptureLigand® human Ig, kappa LC-specific VHH, biotinylated, was immobilized on Streptavidin biosensors and used to capture the Fab fragment of the therapeutic IgG Adalimumab. The Adalimumab Fab fragment was then characterized for its binding kinetics by the titration of its target protein TNFα (HumanKine HZ-1014; 7 to 62 nM;a ssociation for 600 s followed by dissociation for 600 s). The resulting dissociation constant KD of 0.7 nM is consistent with literature values.

Capture of therapeutic IgG (Biolayer Interferometry)
The Nano-CaptureLigand™ human Ig, kappa LC-specific VHH, biotinylated, was immobilized on Streptavidin biosensors and used to capture therapeutic human IgGs (100 nM each) of varying formats (see below). All human IgGs with a κ light chain were successfully captured, while IgG λ was not. Association for 300 s followed by dissociation for 300 s.