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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUP98-NUP96 Polyclonal antibody (12329-1-AP)

    WB analysis using 12329-1-AP (same clone as 12329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12329-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NUP98-NUP96 Polyclonal antibody (12329-1-AP)

    WB analysis of HeLa using 12329-1-AP (same clone as 12329-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12329-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using NUP98-NUP96 Polyclonal antibody (12329-1-AP)

    WB analysis of K-562 using 12329-1-AP (same clone as 12329-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12329-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using NUP98-NUP96 Polyclonal antibody (12329-1-AP)

    WB analysis of Jurkat using 12329-1-AP (same clone as 12329-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12329-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using NUP98-NUP96 Polyclonal antibody (12329-1-AP)

    IP experiment of COLO 320 using 12329-1-AP (same clone as 12329-1-PBS)

    IP result of anti-NUP98-NUP96 (IP:12329-1-AP, 4ug; Detection:12329-1-AP 1:1000) with COLO 320 cells lysate 2000ug. This data was developed using the same antibody clone with 12329-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NUP98-NUP96 Polyclonal antibody (12329-1-AP)

    IHC staining of human breast cancer using 12329-1-AP (same clone as 12329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12329-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NUP98-NUP96 Polyclonal antibody (12329-1-AP)

    IHC staining of human breast cancer using 12329-1-AP (same clone as 12329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12329-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NUP98-NUP96 Polyclonal antibody (12329-1-AP)

    IHC staining of human breast cancer using 12329-1-AP (same clone as 12329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12329-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NUP98-NUP96 Polyclonal antibody (12329-1-AP)

    IHC staining of human breast cancer using 12329-1-AP (same clone as 12329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12329-1-AP (NUP98-NUP96 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12329-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NUP98-NUP96 Polyclonal antibody (12329-1-AP)

    IF Staining of MCF-7 using 12329-1-AP (same clone as 12329-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NUP98-NUP96 antibody (12329-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12329-1-PBS in a different storage buffer formulation.

NUP98-NUP96 Polyclonal antibody, PBS Only

NUP98-NUP96 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12329-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12329-1-PBS targets NUP98-NUP96 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3016

Product name: Recombinant human NUP98-NUP96 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1515-1817 aa of BC012906

Sequence: TADPLDYRLSWHLWEVLRALNYTHLSAQCEGVLQASYAGQLESEGLWEWAIFVLLHIDNSGIREKAVRELLTRHCQLLETPESWAKETFLTQKLRVPAKWIHEAKAVRAHMESDKHLEALCLFKAEHWNRCHKLIIRHLASDAIINENYDYLKGFLEDLAPPERSSLIQDWETSGLVYLDYIRVIEMLRHIQQVDCSGNDLEQLHIKVTSLCSRIEQIQCYSAKDRLAQSDMAKRVANLLRVVLSLHHPPDRTSDSTPDPQRVPLRLLAPHIGRLPMPEDYAMDELRSLTQSYLRELAVGSL

相同性解析による交差性が予測される生物種
Full Name nucleoporin 98kDa
Calculated molecular weight 98 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC012906
Gene Symbol NUP98
Gene ID (NCBI) 4928
RRIDAB_10973678
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP52948
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Nup98-Nup96 (also known as Nup196, ADIR2 or ADAR2), encoded by NUP98 gene, is a component of the nuclear pore complex (NPC) . It is first synthesized as 186-198 kDa Nup98-Nup96 precursors and the precursors shortly undergo autoproteolysis to give rise to two nucleoporins, the amino-terminal Nup98 (Nup98-N) and carboxy-terminal Nup96 (Nup96-C). Both of Nup98 and Nup96 are localized to the nucleoplasmic side of the NPC. This antibody was raised against the carboxy-terminal region of Nup98-Nup96, it recognizes all Nup98-Nup96 proteins except Nup98-N. Since the autoproteolysis is very rapid this antibody usually detected 98-115 kDa Nup96 protein in multiple cell lines. It is notable that RNA-editing deaminase 1 (Gene ID: 104), also named ADAR2, is a different protein from Nup98-Nup96 (ADAR2).

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