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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated NUP50 Monoclonal antibody

NUP50 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D5A7

Cat no : CL488-67001

Synonyms

50 kDa nucleoporin, nucleoporin 50kDa, Nucleoporin Nup50, NUP50

Validation Data Gallery

Applications:
  • FC experiment of HL-60 using CL488-67001

    FC experiment of HL-60 using CL488-67001

    1X10^6 HL-60 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NUP50 (CL488-67001, Clone:1D5A7) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHL-60 cells
Planning an IHC experiment? We recommend our IHCeasy NUP50 Ready-To-Use IHC Kit. NUP50 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67001-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67001 targets NUP50 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NUP50 fusion protein Ag15144 相同性解析による交差性が予測される生物種
Full Name nucleoporin 50kDa
Calculated molecular weight 468 aa, 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC028125
Gene symbol NUP50
Gene ID (NCBI) 10762
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nup50 (also known as Npap60) is a nucleoporin that binds directly to importin α and their interaction plays an important role in nuclear import. Nup50 is a mobile nucleoporin localized primarily in the nuclear basket of the nuclear pore complex (NPC) and in the nucleoplasm. Human Nup50 has two isoforms: Npap60L (1-469 aa), and its alternatively spliced isoform, Npap60S (29-469 aa) of Npap60L.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 NUP50 antibody CL488-67001Download protocol
Standard Protocols
Click here to view our Standard Protocols