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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated NSD3 Polyclonal antibody

NSD3 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-11345

Synonyms

DKFZp667H044, FLJ20353, NSD3, pp14328, Protein whistle, WHSC1 like protein 1, WHSC1L1

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL647-11345

    FC experiment of HeLa using CL647-11345

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human NSD3 (CL647-11345) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA .

Tested Applications

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 11345-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL647-11345 targets NSD3 in FC (Intra) applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NSD3 fusion protein Ag1894 相同性解析による交差性が予測される生物種
Full Name Wolf-Hirschhorn syndrome candidate 1-like 1
Calculated molecular weight 1437 aa, 162 kDa
Observed molecular weight 160-170 kDa, 68-72 kDa
GenBank accession numberBC012059
Gene symbol WHSC1L1
Gene ID (NCBI) 54904
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WHSC1L1, also named as NSD3 and DC28, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is histone methyltransferase. WHSC1L1 is a potently transforming oncogenes based on the number of altered phenotypes expressed by the cells. It contains a PWWP-domain that is a methyl-lysine recognition motif involved in histone code modification and epigenetic regulation of gene expression. Knockdown of WHSC1L1 in 8p11-12-amplified breast cancer cells resulted in profound loss of growth and survival of these cells. (PMID: 20940404). This antibody recognize the long isoform (160-170 kDa) and short isoform (68-72 kDa).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 NSD3 antibody CL647-11345Download protocol
Standard Protocols
Click here to view our Standard Protocols