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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NRF1/nuclear respiratory factor 1 Recombinant mono (83092-1-RR)

    WB analysis using 83092-1-RR (same clone as 83092-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83092-1-RR (NRF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83092-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NRF1/nuclear respiratory factor 1 Recombinant mono (83092-1-RR)

    WB analysis of HeLa using 83092-1-RR (same clone as 83092-1-PBS)

    WB result of NRF1/nuclear respiratory factor 1 antibody (83092-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NRF1/nuclear respiratory factor 1 transfected HeLa cells. This data was developed using the same antibody clone with 83092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical squamous cancer tissue using NRF1/nuclear respiratory factor 1 Recombinant mono (83092-1-RR)

    IHC staining of human cervical squamous cancer using 83092-1-RR (same clone as 83092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 83092-1-RR (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83092-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NRF1/nuclear respiratory factor 1 Recombinant mono (83092-1-RR)

    IF Staining of HepG2 using 83092-1-RR (same clone as 83092-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NRF1/nuclear respiratory factor 1 antibody (83092-1-RR, Clone: 230394B1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83092-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293T cells using NRF1/nuclear respiratory factor 1 Recombinant mono (83092-1-RR)

    FC experiment of HEK-293T using 83092-1-RR (same clone as 83092-1-PBS)

    1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Anti-Human NRF1 (83092-1-RR, Clone:230394B1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83092-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83092-1-RR

    Affinity and Kinetic Characterization of 83092-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83092-1-RR against Human NRF1/nuclear respiratory factor 1 were performed. The affinity constant is 0.115 nM.

  • Featured Product
  • KD/KO Validated

NRF1/nuclear respiratory factor 1 Recombinant monoclonal antibody, PBS Only

NRF1/nuclear respiratory factor 1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83092-1-PBS
Clone No.230394B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

NRF1, nuclear respiratory factor 1, 230394B1, ALPHA PAL, Alpha palindromic-binding protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83092-1-PBS targets NRF1/nuclear respiratory factor 1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3172

Product name: Recombinant human NRF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 213-522 aa of BC016925

Sequence: LKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQQTQTQATATHSIAHLVPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSEAAAHAVATLAEATLQGGGQIVLSGETAAAVGALTGVQDANGLFMADRAGRKWILTDKATGLVQIPVSMYQTVVTSLAQGNGPVQVAMAPVTTRISDSAVTMDGQAVEVVTLEQ

相同性解析による交差性が予測される生物種
Full Name nuclear respiratory factor 1
Calculated molecular weight 522 aa, 56 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC016925
Gene Symbol NRF1
Gene ID (NCBI) 4899
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ16656
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

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