Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, RAW 264.7 cells, NIH/3T3 cells, U2OS cells |
| Positive IHC detected in | human cervical squamous cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83092-1-RR targets NRF1/nuclear respiratory factor 1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag3172 Product name: Recombinant human NRF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 213-522 aa of BC016925 Sequence: LKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQQTQTQATATHSIAHLVPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSEAAAHAVATLAEATLQGGGQIVLSGETAAAVGALTGVQDANGLFMADRAGRKWILTDKATGLVQIPVSMYQTVVTSLAQGNGPVQVAMAPVTTRISDSAVTMDGQAVEVVTLEQ 相同性解析による交差性が予測される生物種 |
| Full Name | nuclear respiratory factor 1 |
| Calculated molecular weight | 522 aa, 56 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC016925 |
| Gene Symbol | NRF1 |
| Gene ID (NCBI) | 4899 |
| RRID | AB_3670806 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16656 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NRF1/nuclear respiratory factor 1 antibody 83092-1-RR | Download protocol |
| IHC protocol for NRF1/nuclear respiratory factor 1 antibody 83092-1-RR | Download protocol |
| WB protocol for NRF1/nuclear respiratory factor 1 antibody 83092-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
