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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NRBF2 Polyclonal antibody (24858-1-AP)

    WB analysis using 24858-1-AP (same clone as 24858-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24858-1-AP (NRBF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24858-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using NRBF2 Polyclonal antibody (24858-1-AP)

    IP experiment of HEK-293 using 24858-1-AP (same clone as 24858-1-PBS)

    IP result of anti-NRBF2 (IP:24858-1-AP, 4ug; Detection:24858-1-AP 1:2000) with HEK-293 cells lysate 2640ug. This data was developed using the same antibody clone with 24858-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NRBF2 Polyclonal antibody (24858-1-AP)

    IHC staining of human liver using 24858-1-AP (same clone as 24858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24858-1-AP (NRBF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24858-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NRBF2 Polyclonal antibody (24858-1-AP)

    IHC staining of human liver using 24858-1-AP (same clone as 24858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24858-1-AP (NRBF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24858-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NRBF2 Polyclonal antibody (24858-1-AP)

    IF Staining of HepG2 using 24858-1-AP (same clone as 24858-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NRBF2 antibody (24858-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24858-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

NRBF2 Polyclonal antibody, PBS Only

NRBF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 24858-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Nuclear receptor-binding factor 2, NRBF-2, NRBF 2, COPR2, COPR1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24858-1-PBS targets NRBF2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRBF2 fusion protein Ag21403 相同性解析による交差性が予測される生物種
Full Name nuclear receptor binding factor 2
Calculated molecular weight 32 kDa
Observed molecular weight 35-37 kDa
GenBank accession numberBC001345
Gene Symbol NRBF2
Gene ID (NCBI) 29982
RRIDAB_2879759
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96F24
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NRBF2 also termed as Nuclear receptor-binding factor 2or COPR is a 287 amino acid protein, which Interacts with PPARA, PPARD and PPARG. NRBF2 as transcription activator may modulate transcriptional activation by target nuclear receptors. NRBF2 is expressed in keratinocytes, liver and placenta. NRBF2, which exists as two isoforms due to alternative splicing, is localized to both the nucleus and the cytoplasm.