Validation Data Gallery
Tested Applications
| Positive WB detected in | pig retina tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
HRP-66494 targets NR2E3 in WB applications and shows reactivity with Pig samples.
| Tested Reactivity | Pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5831 Product name: Recombinant human NR2E3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-322 aa of BC041421 Sequence: MCPVDKAHRNQCQACRLKKCLQAGMNQDAVQNERQPRSTAQVHLDSMESNTESRPESLVAPPAPAGRSPRGPTPMSAARALGHHFMASLITAETCAKLEPEDADENIDVTSNDPEFPSSPYSSSSPCGLDSIHETSARLLFMAVKWAKNLPVFSSLPFRDQVILLEEAWSELFLLGAIQWSLPLDSCPLLAPPEASAAGGAQGRLTLASMETRVLQETISRFRALAVDPTEFACMKALVLFKPETRGLKDPEHVEALQDQSQVMLSQHSKAHHPSQPVRFGKLLLLLPSLRFITAERIELLFFRKTIGNTPMEKLLCDMFKN 相同性解析による交差性が予測される生物種 |
| Full Name | nuclear receptor subfamily 2, group E, member 3 |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 45-50 kDa |
| GenBank accession number | BC041421 |
| Gene Symbol | NR2E3 |
| Gene ID (NCBI) | 10002 |
| RRID | AB_2883842 |
| Conjugate | HRP |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9Y5X4 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NR2E3, also known as PNR, encodes a retinal nuclear receptor that is a ligand-dependent transcription factor. This protein is part of a large family of nuclear receptor transcription factors involved in signaling pathways. NR2E3 influences the development of photoreceptors and their differentiation into rod and cone types, and acts as a ranscriptional factor that is an activator of rod development and repressor of cone development [PMID:20725840]. It binds the promoter region of a number of rod- and cone-specific genes, including rhodopsin, M- and S-opsin and rod-specific phosphodiesterase beta subunit. [PMID:15689355]
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP NR2E3 antibody HRP-66494 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
