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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NPRL2 Polyclonal antibody (10157-1-AP)

    WB analysis of HeLa using 10157-1-AP (same clone as 10157-1-PBS)

    WB result of TUSC4 antibody (10157-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TUSC4 transfected HeLa cells. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NPRL2 Polyclonal antibody (10157-1-AP)

    WB analysis using 10157-1-AP (same clone as 10157-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10157-1-AP (NPRL2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse pancreas tissue using NPRL2 Polyclonal antibody (10157-1-AP)

    WB analysis of mouse pancreas using 10157-1-AP (same clone as 10157-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10157-1-AP (TUSC4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse pancreas tissue using NPRL2 Polyclonal antibody (10157-1-AP)

    IP experiment of mouse pancreas using 10157-1-AP (same clone as 10157-1-PBS)

    IP result of anti-NPRL2 (IP:10157-1-AP, 3ug; Detection:10157-1-AP 1:500) with mouse pancreas tissue lysate 4000ug. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NPRL2 Polyclonal antibody (10157-1-AP)

    IHC staining of human lung cancer using 10157-1-AP (same clone as 10157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NPRL2 Polyclonal antibody (10157-1-AP)

    IHC staining of human lung cancer using 10157-1-AP (same clone as 10157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using NPRL2 Polyclonal antibody (10157-1-AP)

    IHC staining of human pancreas using 10157-1-AP (same clone as 10157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using NPRL2 Polyclonal antibody (10157-1-AP)

    IHC staining of human pancreas using 10157-1-AP (same clone as 10157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using NPRL2 Polyclonal antibody (10157-1-AP)

    IHC staining of human skeletal muscle using 10157-1-AP (same clone as 10157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using NPRL2 Polyclonal antibody (10157-1-AP)

    IHC staining of human skeletal muscle using 10157-1-AP (same clone as 10157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 10157-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NPRL2 Polyclonal antibody, PBS Only

NPRL2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10157-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

TUSC4, G21 protein, GATOR1 complex protein NPRL2, Gene 21 protein, Nitrogen permease regulator 2-like protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10157-1-PBS targets NPRL2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0210

Product name: Recombinant human NPR2L,TUSC4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-270 aa of BC002791

Sequence: GCRIECIFFSEFHPTLGPKITYQVPEDFISRELFDTVQVYIITKPELQNKLITVTAMEKKLIGCPVCIEHKKYSRNALLFNLGFVCDAQAKTCALEPIVKKLAGYLTTLELESSFVSMEESKQKLVPIMTILLEELNASGRCTLPIDESNTIHLKVIEQRPDPPVAQEYDVPVFTKDKEDFFNSQWDLTTQQILPYIDGFRHIQKISAEADVELNLVRIAIQNLLYYGVVTLVSILQYSNVYCPTPKVQDLVDDKSLQEACLSYVTKQGH

相同性解析による交差性が予測される生物種
Full Name tumor suppressor candidate 4
Calculated molecular weight 44 kDa
Observed molecular weight40, 44-50 kDa
GenBank accession numberBC002791
Gene Symbol TUSC4
Gene ID (NCBI) 10641
RRIDAB_514025
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WTW4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NPRL2 (NPR2 Like, GATOR1 Complex Subunit) is a Protein Coding gene associated with Epilepsy, Familial Focal. As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. Homologous to yeast nitrogen permease (candidate tumor suppressor) (NM_006545, BC021984) gene encodes a protein of molecular mass of 50 kDa. Data by using overlapping and nested homozygous deletions methods indicate that this protein might be a tumor suppressor involved in the development of lung cancers.

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