Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse pancreas tissue, HeLa cells |
| Positive IP detected in | mouse pancreas tissue |
| Positive IHC detected in | human lung cancer tissue, human pancreas tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
10157-1-AP targets NPRL2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0210 Product name: Recombinant human NPR2L,TUSC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-270 aa of BC002791 Sequence: GCRIECIFFSEFHPTLGPKITYQVPEDFISRELFDTVQVYIITKPELQNKLITVTAMEKKLIGCPVCIEHKKYSRNALLFNLGFVCDAQAKTCALEPIVKKLAGYLTTLELESSFVSMEESKQKLVPIMTILLEELNASGRCTLPIDESNTIHLKVIEQRPDPPVAQEYDVPVFTKDKEDFFNSQWDLTTQQILPYIDGFRHIQKISAEADVELNLVRIAIQNLLYYGVVTLVSILQYSNVYCPTPKVQDLVDDKSLQEACLSYVTKQGH 相同性解析による交差性が予測される生物種 |
| Full Name | tumor suppressor candidate 4 |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 44-50 kDa |
| GenBank accession number | BC002791 |
| Gene Symbol | TUSC4 |
| Gene ID (NCBI) | 10641 |
| RRID | AB_514025 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WTW4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NPRL2 (NPR2 Like, GATOR1 Complex Subunit) is a Protein Coding gene associated with Epilepsy, Familial Focal. As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. Homologous to yeast nitrogen permease (candidate tumor suppressor) (NM_006545, BC021984) gene encodes a protein of molecular mass of 50 kDa. Data by using overlapping and nested homozygous deletions methods indicate that this protein might be a tumor suppressor involved in the development of lung cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NPRL2 antibody 10157-1-AP | Download protocol |
| IP protocol for NPRL2 antibody 10157-1-AP | Download protocol |
| WB protocol for NPRL2 antibody 10157-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Carcinog Downregulation of nitrogen permease regulator like-2 activates PDK1-AKT1 and contributes to the malignant growth of glioma cells. | ||
Cancer Sci TUSC4/NPRL2, a novel PDK1-interacting protein, inhibits PDK1 tyrosine phosphorylation and its downstream signaling.
| ||
Exp Cell Res NPRL2 reduces the niraparib sensitivity of castration-resistant prostate cancer via interacting with UBE2M and enhancing neddylation. | ||
