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Validation Data Gallery

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  • Western Blot (WB) analysis of human adrenal gland tissue using NNT Polyclonal antibody (13442-2-AP)

    WB analysis of human adrenal gland using 13442-2-AP (same clone as 13442-2-PBS)

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using NNT Polyclonal antibody (13442-2-AP)

    WB analysis of human liver using 13442-2-AP (same clone as 13442-2-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using NNT Polyclonal antibody (13442-2-AP)

    WB analysis of human heart using 13442-2-AP (same clone as 13442-2-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human adrenal gland tissue using NNT Polyclonal antibody (13442-2-AP)

    WB analysis of human adrenal gland using 13442-2-AP (same clone as 13442-2-PBS)

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using NNT Polyclonal antibody (13442-2-AP)

    IP experiment of HepG2 using 13442-2-AP (same clone as 13442-2-PBS)

    IP result of anti-NNT (IP:13442-2-AP, 4ug; Detection:13442-2-AP 1:300) with HepG2 cells lysate 2800ug. This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NNT Polyclonal antibody (13442-2-AP)

    IHC staining of human liver cancer using 13442-2-AP (same clone as 13442-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13442-2-AP (NNT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NNT Polyclonal antibody (13442-2-AP)

    IHC staining of human liver cancer using 13442-2-AP (same clone as 13442-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13442-2-AP (NNT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NNT Polyclonal antibody (13442-2-AP)

    IF Staining of HeLa using 13442-2-AP (same clone as 13442-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NNT antibody (13442-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NNT Polyclonal antibody (13442-2-AP)

    IF Staining of HeLa using 13442-2-AP (same clone as 13442-2-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13442-2-AP (NNT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NNT Polyclonal antibody, PBS Only

NNT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13442-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:7.1.1.1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13442-2-PBS targets NNT in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4259

Product name: Recombinant human NNT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 30-236 aa of BC032370

Sequence: MNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLTEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK

相同性解析による交差性が予測される生物種
Full Name nicotinamide nucleotide transhydrogenase
Calculated molecular weight 1085 aa, 114 kDa
Observed molecular weight 114 kDa
GenBank accession numberBC032370
Gene Symbol NNT
Gene ID (NCBI) 23530
RRIDAB_2153596
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13423
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and βsubunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 110 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).

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