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Validation Data Gallery

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  • Western Blot (WB) analysis of human adrenal gland tissue using NNT Polyclonal antibody (13442-2-AP)

    WB analysis of human adrenal gland using 13442-2-AP

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using NNT Polyclonal antibody (13442-2-AP)

    WB analysis of human liver using 13442-2-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using NNT Polyclonal antibody (13442-2-AP)

    WB analysis of human heart using 13442-2-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human adrenal gland tissue using NNT Polyclonal antibody (13442-2-AP)

    WB analysis of human adrenal gland using 13442-2-AP

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using NNT Polyclonal antibody (13442-2-AP)

    IP experiment of HepG2 using 13442-2-AP

    IP result of anti-NNT (IP:13442-2-AP, 4ug; Detection:13442-2-AP 1:300) with HepG2 cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NNT Polyclonal antibody (13442-2-AP)

    IHC staining of human liver cancer using 13442-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13442-2-AP (NNT antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NNT Polyclonal antibody (13442-2-AP)

    IHC staining of human liver cancer using 13442-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13442-2-AP (NNT antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NNT Polyclonal antibody (13442-2-AP)

    IF Staining of HeLa using 13442-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NNT antibody (13442-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NNT Polyclonal antibody (13442-2-AP)

    IF Staining of HeLa using 13442-2-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13442-2-AP (NNT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

NNT Polyclonal antibody

NNT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13442-2-AP
Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

EC:7.1.1.1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman adrenal gland tissue, human heart tissue, human liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13442-2-AP targets NNT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4259

Product name: Recombinant human NNT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 30-236 aa of BC032370

Sequence: MNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLTEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK

相同性解析による交差性が予測される生物種
Full Name nicotinamide nucleotide transhydrogenase
Calculated molecular weight 1085 aa, 114 kDa
Observed molecular weight 114 kDa
GenBank accession numberBC032370
Gene Symbol NNT
Gene ID (NCBI) 23530
RRIDAB_2153596
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13423
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and βsubunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 110 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).

Protocols

Product Specific Protocols
IF protocol for NNT antibody 13442-2-APDownload protocol
IHC protocol for NNT antibody 13442-2-APDownload protocol
IP protocol for NNT antibody 13442-2-APDownload protocol
WB protocol for NNT antibody 13442-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nat Metab

Mitochondrial NADP+ is essential for proline biosynthesis during cell growth.

Authors - Diem H Tran
View Article
mouseWB,IHC

Nat Aging

Inhibiting bridge integrator 2 phosphorylation leads to improved oocyte quality, ovarian health and fertility in aging and after chemotherapy in mice

Authors - Feng-Yu Zhu
View Article
humanWB

Nat Cell Biol

Mitochondrial NADPH fuels mitochondrial fatty acid synthesis and lipoylation to power oxidative metabolism

Authors - Dohun Kim
View Article
human,mouseWB,IHC,IF

Redox Biol

Nicotinamide nucleotide transhydrogenase-mediated redox homeostasis promotes tumor growth and metastasis in gastric cancer.

Authors - Shuai Li
  • KD Validated
View Article
mouseWB

Sci Adv

Chronic stress hinders sensory axon regeneration via impairing mitochondrial cristae and OXPHOS

Authors - Yu Ruan
View Article
mouseWB

Cell Death Dis

ApoM maintains cellular homeostasis between mitophagy and apoptosis by affecting the stability of Nnt mRNA through the Zic3-ApoM-Elavl2-Nnt axis during neural tube closure

Authors - Qing Liu
View Article
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