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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NLRP3 Polyclonal antibody (27458-1-AP)

    WB analysis using 27458-1-AP (same clone as 27458-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27458-1-AP (NLRP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27458-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using NLRP3 Polyclonal antibody (27458-1-AP)

    WB analysis of THP-1 using 27458-1-AP (same clone as 27458-1-PBS)

    WB result of NLRP3 antibody (27458-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NLRP3 transfected THP-1 cells. This data was developed using the same antibody clone with 27458-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of THP-1 cells using NLRP3 Polyclonal antibody (27458-1-AP)

    IP experiment of THP-1 using 27458-1-AP (same clone as 27458-1-PBS)

    IP result of anti-NLRP3 (IP:27458-1-AP, 4ug; Detection:27458-1-AP 1:800) with THP-1 cells lysate 1520 ug. This data was developed using the same antibody clone with 27458-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NLRP3 Polyclonal antibody (27458-1-AP)

    IF Staining of HepG2 using 27458-1-AP (same clone as 27458-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NLRP3 antibody (27458-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 27458-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NLRP3 Polyclonal antibody, PBS Only

NLRP3 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 27458-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Angiotensin/vasopressin receptor AII/AVP-like, C1orf7, CIAS1, Cold-induced autoinflammatory syndrome 1 protein, EC:3.6.4.-

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27458-1-PBS targets NLRP3 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26272

Product name: Recombinant human NLRP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 937-1036 aa of NM_001079821

Sequence: MLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

相同性解析による交差性が予測される生物種
Full Name NLR family, pyrin domain containing 3
Calculated molecular weight 118 kDa
Observed molecular weight110 kDa
GenBank accession numberNM_001079821
Gene Symbol NLRP3
Gene ID (NCBI) 114548
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96P20
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NLRP3, also named as NALP3, CIASI, C1orf7, PYPAF1, Cryopyrin and MIM606416, belongs to NLR family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NLRP3 inflammasome is expressed in immune cells, including monocytes, macrophages, and dendritic cells. When the NLRP3 inflammasome is activated, the PYD domain of NLRP3 mediates recruitment of an adaptor protein called ASC and the effector protein procaspase-1 to form an NLRP3 inflammasome complex that can cleave inactive procaspase-1 to from active caspase-1. And then the active caspase-1 results in secretion of interleukin (IL)-18 and IL-1β. Activation of the NLRP3 inflammasome is also required for HMGB1 secretion. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death(PMID:27669650). NLRP3 has some isoforms with the MW of 106-118 kDa and 75-83 kDa.

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