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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using NLGN1 Monoclonal antibody (66964-1-Ig)

    WB analysis of mouse brain using 66964-1-Ig (same clone as 66964-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using NLGN1 Monoclonal antibody (66964-1-Ig)

    WB analysis of rat brain using 66964-1-Ig (same clone as 66964-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit brain tissue using NLGN1 Monoclonal antibody (66964-1-Ig)

    WB analysis of rabbit brain using 66964-1-Ig (same clone as 66964-1-PBS)

    rabbit brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using NLGN1 Monoclonal antibody (66964-1-Ig)

    WB analysis of pig brain using 66964-1-Ig (same clone as 66964-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NLGN1 Monoclonal antibody (66964-1-Ig)

    WB analysis using 66964-1-Ig (same clone as 66964-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66964-1-Ig (NLGN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NLGN1 Monoclonal antibody (66964-1-Ig)

    IHC staining of mouse brain using 66964-1-Ig (same clone as 66964-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66964-1-Ig (NLGN1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NLGN1 Monoclonal antibody (66964-1-Ig)

    IHC staining of mouse brain using 66964-1-Ig (same clone as 66964-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66964-1-Ig (NLGN1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66964-1-PBS in a different storage buffer formulation.

NLGN1 Monoclonal antibody, PBS Only

NLGN1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66964-1-PBS
Clone No.2D1A12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, Indirect ELISA

Synonyms

NLG1, KIAA1070, neuroligin 1, Neuroligin-1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66964-1-PBS targets NLGN1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6356

Product name: Recombinant human NLGN1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 40-406 aa of BC032555

Sequence: AGHVLSQKLDDVDPLVATNFGKIRGIKKELNNEILGPVIQFLGVPYAAPPTGERRFQPPEPPSPWSDIRNATQFAPVCPQNIIDGRLPEVMLPVWFTNNLDVVSSYVQDQSEDCLYLNIYVPTEDDIRDSGGPKPVMVYIHGGSYMEGTGNLYDGSVLASYGNVIVITVNYRLGVLGFLSTGDQAAKGNYGLLDLIQALRWTSENIGFFGGDPLRITVFGSGAGGSCVNLLTLSHYSEGNRWSNSTKGLFQRAIAQSGTALSSWAVSFQPAKYARMLATKVGCNVSDTVELVECLQKKPYKELVDQDIQPARYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGLKFVENIVDSDDGI

相同性解析による交差性が予測される生物種
Full Name neuroligin 1
Calculated molecular weight 840 aa, 94 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC032555
Gene Symbol NLGN1
Gene ID (NCBI) 22871
RRIDAB_2882287
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ8N2Q7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NLGN1, also named KIAA1070, belongs to the type-B carboxylesterase/lipase family. Neuroligin1 (NLGN1) is a main component of the excitatory glutamatergic synapses complex and plays a role in synapse assembly and function (PMID: 34340665). NLGN1 has 2 isoforms with the MV of 92 and 96 kDa. Sometimes higher molecular weight of around 116 kDa can also be observed, which is a glycosylated mature form of NLGN1 (PMID: 28841651).

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