Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue, rabbit brain tissue, pig brain tissue, human brain tissue, pig cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:750-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
Product Information
66964-1-Ig targets NLGN1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.
| Tested Reactivity | Human, Mouse, Rat, Pig, Rabbit |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NLGN1 fusion protein Ag6356 相同性解析による交差性が予測される生物種 |
| Full Name | neuroligin 1 |
| Calculated molecular weight | 840 aa, 94 kDa |
| Observed molecular weight | 116 kDa |
| GenBank accession number | BC032555 |
| Gene Symbol | NLGN1 |
| Gene ID (NCBI) | 22871 |
| RRID | AB_2882287 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8N2Q7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NLGN1, also named KIAA1070, belongs to the type-B carboxylesterase/lipase family. Neuroligin1 (NLGN1) is a main component of the excitatory glutamatergic synapses complex and plays a role in synapse assembly and function (PMID: 34340665). NLGN1 has 2 isoforms with the MV of 92 and 96 kDa. Sometimes higher molecular weight of around 116 kDa can also be observed, which is a glycosylated mature form of NLGN1 (PMID: 28841651).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NLGN1 antibody 66964-1-Ig | Download protocol |
| IHC protocol for NLGN1 antibody 66964-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neuron Longitudinal single-cell transcriptional dynamics throughout neurodegeneration in SCA1 | ||
Neuroscience Activity-dependent Synaptic Recruitment of Neuroligin 1 in Spinal Dorsal Horn Contributed to Inflammatory Pain. | ||
J Ethnopharmacol Multimodal investigation reveals the neuroprotective mechanism of Angong Niuhuang pill for intracerebral hemorrhage: Converging bioinformatics, network pharmacology, and experimental validation | ||
Aging (Albany NY) Metastasis-related gene signature associates with immunity and predicts prognosis accurately in patients with osteosarcoma | ||
Neurochem Int Mitochondrial protein prohibitin promotes learning memory recovery in mice following intracerebral hemorrhage via CAMKII/CRMP signaling pathway | ||
Aging (Albany NY) Longitudinal characterization of behavioral, morphological and transcriptomic changes in a tauopathy mouse model |