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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NHLRC2 Polyclonal antibody (29416-1-AP)

    WB analysis using 29416-1-AP (same clone as 29416-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29416-1-AP (NHLRC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29416-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NHLRC2 Polyclonal antibody (29416-1-AP)

    IHC staining of mouse brain using 29416-1-AP (same clone as 29416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29416-1-AP (NHLRC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29416-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NHLRC2 Polyclonal antibody (29416-1-AP)

    IHC staining of mouse brain using 29416-1-AP (same clone as 29416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29416-1-AP (NHLRC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29416-1-PBS in a different storage buffer formulation.

NHLRC2 Polyclonal antibody, PBS Only

NHLRC2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29416-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29416-1-PBS targets NHLRC2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30159

Product name: Recombinant human NHLRC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 43-200 aa of NM_198514

Sequence: QKVDGWEQDLSVPEFPEGLEWLNTEEPISVYKDLCGKIVVLDFFTYCCINCIHLLPDLHALEHTYSDKDGLLIIGVHSAKFPNEKVLDNIKSAVLRYNITHPMVNDADASLWQELEVSCWPTLVILGPRGNMLFSLIGEGHKDKLFLYTSIALKYYKD

相同性解析による交差性が予測される生物種
Full Name NHL repeat containing 2
Calculated molecular weight 79 kDa
Observed molecular weight80 kDa
GenBank accession numberNM_198514
Gene Symbol NHLRC2
Gene ID (NCBI) 374354
RRIDAB_2918302
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NBF2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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