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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NHLRC2 Polyclonal antibody

NHLRC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29416-1-AP

Synonyms

DKFZp779F115, FLJ20147, FLJ25621, FLJ33312, NHL repeat containing 2, NHLRC2

Validation Data Gallery

Applications:
  • WB analysis using 29416-1-AP

    WB analysis using 29416-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29416-1-AP (NHLRC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 29416-1-AP

    IHC staining of mouse brain using 29416-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29416-1-AP (NHLRC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 29416-1-AP

    IHC staining of mouse brain using 29416-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29416-1-AP (NHLRC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inU-251 cells, mouse spleen tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

29416-1-AP targets NHLRC2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NHLRC2 fusion protein Ag30159 相同性解析による交差性が予測される生物種
Full Name NHL repeat containing 2
Calculated molecular weight 79 kDa
Observed molecular weight80 kDa
GenBank accession numberNM_198514
Gene symbol NHLRC2
Gene ID (NCBI) 374354
RRIDAB_2918302
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NHLRC2 antibody 29416-1-APDownload protocol
IHC protocol for NHLRC2 antibody 29416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols