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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NFIC Monoclonal antibody (68318-1-Ig)

    WB analysis using 68318-1-Ig (same clone as 68318-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68318-1-Ig (NFIC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68318-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NFIC Monoclonal antibody (68318-1-Ig)

    WB analysis using 68318-1-Ig (same clone as 68318-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68318-1-Ig (NFIC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68318-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using NFIC Monoclonal antibody (68318-1-Ig)

    FC experiment of HeLa using 68318-1-Ig (same clone as 68318-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NFIC Monoclonal antibody (68318-1-Ig, Clone:1A11A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68318-1-PBS in a different storage buffer formulation.

NFIC Monoclonal antibody, PBS Only

NFIC Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 68318-1-PBS
Clone No.1A11A5

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

NF-I/C, NF1-C, NF I, CTF, CCAAT-box-binding transcription factor

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68318-1-PBS targets NFIC in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9472

Product name: Recombinant human NFIC protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-351 aa of BC012120

Sequence: MYSSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKPEVKQKWASRLLAKLRKDIRPECREDFVLSITGKKAPGCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKAAQCGHPVLCVQPHHIGVAVKELDLYLAYFVRERDAEQSGSPRTGMGSDQEDSKPITLDTTDFQESFVTSGVFSVTELIQVSRTPVVTGTGPNFSLGELQGHLAYDLNPASTGLRRTLPSTSSSGSKRHKSGSMEEDVDTSPGGDYYTSPSSPTSSSRNWTEDMEGGISSPVKKTEMDKSPFNSPSPQDSPRLSSFTQ

相同性解析による交差性が予測される生物種
Full Name nuclear factor I/C (CCAAT-binding transcription factor)
Calculated molecular weight428aa,48 kDa; 508aa,57 kDa
Observed molecular weight 50-56 kDa
GenBank accession numberBC012120
Gene Symbol NFIC
Gene ID (NCBI) 4782
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP08651
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NFIC, also named as Nuclear factor 1 C-type, is a 508 amino acid protein, which contains 1 CTF/NF-I DNA-binding domain and belongs to the CTF/NF-I family. NFIC localizes in the nucleus and recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

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