Validation Data Gallery
Tested Applications
| Positive WB detected in | HSC-T6 cells, Saos-2 cells, U2OS cellls, LNCaP cells, HeLa cells, NIH/3T3 cells, 4T1 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68318-1-Ig targets NFIC in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9472 Product name: Recombinant human NFIC protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-351 aa of BC012120 Sequence: MYSSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKPEVKQKWASRLLAKLRKDIRPECREDFVLSITGKKAPGCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKAAQCGHPVLCVQPHHIGVAVKELDLYLAYFVRERDAEQSGSPRTGMGSDQEDSKPITLDTTDFQESFVTSGVFSVTELIQVSRTPVVTGTGPNFSLGELQGHLAYDLNPASTGLRRTLPSTSSSGSKRHKSGSMEEDVDTSPGGDYYTSPSSPTSSSRNWTEDMEGGISSPVKKTEMDKSPFNSPSPQDSPRLSSFTQ 相同性解析による交差性が予測される生物種 |
| Full Name | nuclear factor I/C (CCAAT-binding transcription factor) |
| Calculated molecular weight | 428aa,48 kDa; 508aa,57 kDa |
| Observed molecular weight | 50-56 kDa |
| GenBank accession number | BC012120 |
| Gene Symbol | NFIC |
| Gene ID (NCBI) | 4782 |
| RRID | AB_3085054 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08651 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NFIC, also named as Nuclear factor 1 C-type, is a 508 amino acid protein, which contains 1 CTF/NF-I DNA-binding domain and belongs to the CTF/NF-I family. NFIC localizes in the nucleus and recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for NFIC antibody 68318-1-Ig | Download protocol |
| WB protocol for NFIC antibody 68318-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |