• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    WB analysis of HeLa using 21686-1-AP (same clone as 21686-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    WB analysis using 21686-1-AP (same clone as 21686-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    WB analysis of SH-SY5Y using 21686-1-AP (same clone as 21686-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    WB analysis of NIH/3T3 using 21686-1-AP (same clone as 21686-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    WB analysis of HepG2 using 21686-1-AP (same clone as 21686-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    WB analysis of MCF-7 using 21686-1-AP (same clone as 21686-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    WB analysis of HEK-293 using 21686-1-AP (same clone as 21686-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    WB analysis of Jurkat using 21686-1-AP (same clone as 21686-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    IP experiment of HeLa using 21686-1-AP (same clone as 21686-1-PBS)

    IP result of anti-NF2 (IP:21686-1-AP, 4ug; Detection:21686-1-AP 1:5000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human meningioma tissue using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    IHC staining of human meningioma using 21686-1-AP (same clone as 21686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human meningioma tissue using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    IHC staining of human meningioma using 21686-1-AP (same clone as 21686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    IF Staining of HeLa using 21686-1-AP (same clone as 21686-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NF2 antibody (21686-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Neurofibromin 2 Polyclonal antibody (21686-1-AP)

    IF Staining of HeLa using 21686-1-AP (same clone as 21686-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21686-1-AP (NF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Neurofibromin 2 Polyclonal antibody, PBS Only

Neurofibromin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21686-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

NF2, Merlin, Moesin-ezrin-radixin-like protein, Neurofibromin-2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21686-1-PBS targets Neurofibromin 2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16265

Product name: Recombinant human NF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 245-595 aa of BC020257

Sequence: DPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPAPLPPDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHNENSDRGGSSKHNTIKKLTLQSAKSRVAFFEEL

相同性解析による交差性が予測される生物種
Full Name neurofibromin 2 (merlin)
Calculated molecular weight 595 aa, 70 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC020257
Gene Symbol NF2
Gene ID (NCBI) 4771
RRIDAB_10792410
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP35240
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors.

||
New chat

Able™

正在加载，请稍候...