NF2 Polyclonal antibody
NF2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, canine
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 21686-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, HEK-293 cells, Jurkat cells, SH-SY5Y cells, NIH/3T3 cells, MDCK cells, mouse brain tissue, rat brain tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | mouse brain tissue, human meningioma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:750-1:3000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 5 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
21686-1-AP targets NF2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
Tested Reactivity | human, mouse, rat, canine |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NF2 fusion protein Ag16265 |
Full Name | neurofibromin 2 (merlin) |
Calculated Molecular Weight | 595 aa, 70 kDa |
Observed Molecular Weight | 66-70 kDa |
GenBank Accession Number | BC020257 |
Gene Symbol | NF2 |
Gene ID (NCBI) | 4771 |
RRID | AB_10792410 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for NF2 antibody 21686-1-AP | Download protocol |
IHC protocol for NF2 antibody 21686-1-AP | Download protocol |
IF protocol for NF2 antibody 21686-1-AP | Download protocol |
IP protocol for NF2 antibody 21686-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Thyroid MicroRNA-146b-3p promotes cell metastasis by directly targeting NF2 in human papillary thyroid cancer. | ||
Onco Targets Ther PWP1 Promotes the Malignant Phenotypes of Lung Cancer Cells by Interacting with DVL2 and Merlin.
| ||
Cancer Manag Res MiR-25-3p Serves as an Oncogenic MicroRNA by Downregulating the Expression of Merlin in Osteosarcoma. | ||
Biochem Biophys Res Commun A hairpin within YAP mRNA 3'UTR functions in regulation at post-transcription level. | ||
Arch Pathol Lab Med Eosinophilic Solid and Cystic Renal Cell Carcinoma: Morphologic and Immunohistochemical Study of 18 Cases and Review of the Literature |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Bettina (Verified Customer) (08-04-2022) | Worked well
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