- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated NF-κB p65 Polyclonal antibody
NF-κB p65 Polyclonal Antibody for IF
Host / Isotype
Rabbit / IgG
human, mouse, rat, pig
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-10745
Validation Data Gallery
|Positive IF detected in
|IF : 1:50-1:500
|Sample-dependent, check data in validation data gallery
CL488-10745 targets NF-κB p65 in IF applications and shows reactivity with human, mouse, rat, pig samples.
|human, mouse, rat, pig
|Host / Isotype
|Rabbit / IgG
|NF-κB p65 fusion protein Ag1199 相同性解析による交差性が予測される生物種
|v-rel reticuloendotheliosis viral oncogene homolog A (avian)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|CoraLite® Plus 488 Fluorescent Dye
|Excitation/Emission maxima wavelengths
|493 nm / 522 nm
|Antigen affinity purification
|PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
|Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.
Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.