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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NESP55,GNAS Recombinant monoclonal antibody (81802-2-RR)

    WB analysis using 81802-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81802-2-RR (NESP55,GNAS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using NESP55,GNAS Recombinant monoclonal antibody (81802-2-RR)

    WB analysis of MCF-7 using 81802-2-RR

    WB result of NESP55,GNAS antibody (81802-2-RR; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NESP55,GNAS transfected MCF-7 cells.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NESP55,GNAS Recombinant monoclonal antibody (81802-2-RR)

    IF Staining of MCF-7 using 81802-2-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NESP55,GNAS antibody (81802-2-RR, Clone: 251463D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 81802-2-RR

    Affinity and Kinetic Characterization of 81802-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81802-2-RR against Human NESP55,GNAS were performed. The affinity constant is below 1 pM.

  • KD/KO Validated

NESP55,GNAS Recombinant monoclonal antibody

NESP55,GNAS Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 81802-2-RR
Clone No.251463D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

GNAS, GNAS1, GPIPIRRH peptide, LHAL tetrapeptide, NESP55

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, MCF-7 cells, Jurkat cells, MCGF-7 cells, mouse liver tissue, rat liver tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81802-2-RR targets NESP55,GNAS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0197

Product name: Recombinant human NESP55,GNAS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 230-394 aa of BC002722

Sequence: ERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

相同性解析による交差性が予測される生物種
Full Name GNAS complex locus
Calculated molecular weight45 kDa
Observed molecular weight46 kDa
GenBank accession numberBC002722
Gene Symbol GNAS
Gene ID (NCBI) 2778
RRIDAB_3743396
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP63092
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The near two bands represent two different isoforms. The expression profile/molecular weight observed are consistent with what has been described in the literature (PMID : 3258290).

Protocols

Product Specific Protocols
IF protocol for NESP55,GNAS antibody 81802-2-RRDownload protocol
WB protocol for NESP55,GNAS antibody 81802-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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