Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
81802-2-PBS targets NESP55,GNAS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag0197 Product name: Recombinant human NESP55,GNAS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 230-394 aa of BC002722 Sequence: ERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL 相同性解析による交差性が予測される生物種 |
| Full Name | GNAS complex locus |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | BC002722 |
| Gene Symbol | GNAS |
| Gene ID (NCBI) | 2778 |
| RRID | AB_3743396 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P63092 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The near two bands represent two different isoforms. The expression profile/molecular weight observed are consistent with what has been described in the literature (PMID : 3258290).