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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NESG1 Polyclonal antibody (21077-1-AP)

    WB analysis using 21077-1-AP (same clone as 21077-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21077-1-AP (NESG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21077-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using NESG1 Polyclonal antibody (21077-1-AP)

    WB analysis of rat testis using 21077-1-AP (same clone as 21077-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 21077-1-AP (NESG1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21077-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using NESG1 Polyclonal antibody (21077-1-AP)

    WB analysis of mouse testis using 21077-1-AP (same clone as 21077-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21077-1-AP (NESG1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21077-1-PBS in a different storage buffer formulation.

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NESG1 Polyclonal antibody, PBS Only

NESG1 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 21077-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21077-1-PBS targets NESG1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NESG1 fusion protein Ag15257 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 19
Calculated molecular weight 551 aa, 66 kDa
Observed molecular weight66 kDa
GenBank accession numberBC027874
Gene Symbol CCDC19
Gene ID (NCBI) 25790
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UL16
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CCDC19, also named as NESG1, is specifically expressed in human nasopharynx and trachea, and decreased or absent in nasopharyngeal carcinoma (NPC) tissues and cell lines. In addition, the levels of NESG1 protein are significantly greater in the low-grade NPC tissues than that in the high-grade NPC tissues. NESG1 also significantly regulats the expression of cell cycle regulators CCNA1 and p21. NESG1 may act as a tumor suppressor by inhibiting cell proliferation, invasion and migration of NPC cells.