Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
21077-1-AP targets NESG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NESG1 fusion protein Ag15257 相同性解析による交差性が予測される生物種 |
| Full Name | coiled-coil domain containing 19 |
| Calculated molecular weight | 551 aa, 66 kDa |
| Observed molecular weight | 66 kDa |
| GenBank accession number | BC027874 |
| Gene Symbol | CCDC19 |
| Gene ID (NCBI) | 25790 |
| RRID | AB_3669356 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UL16 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCDC19, also named as NESG1, is specifically expressed in human nasopharynx and trachea, and decreased or absent in nasopharyngeal carcinoma (NPC) tissues and cell lines. In addition, the levels of NESG1 protein are significantly greater in the low-grade NPC tissues than that in the high-grade NPC tissues. NESG1 also significantly regulats the expression of cell cycle regulators CCNA1 and p21. NESG1 may act as a tumor suppressor by inhibiting cell proliferation, invasion and migration of NPC cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NESG1 antibody 21077-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis Loss of connective tissue growth factor as an unfavorable prognosis factor activates miR-18b by PI3K/AKT/C-Jun and C-Myc and promotes cell growth in nasopharyngeal carcinoma. | ||
J Cell Mol Med Candidate tumour suppressor CCDC19 regulates miR-184 direct targeting of C-Myc thereby suppressing cell growth in non-small cell lung cancers.
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