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Validation Data Gallery

  • NEDD8-Trap Agarose (MG-3002A) was used to immunoprecipitate endogenous NEDD8 and NEDDylated proteins from human HEPG2 cells. Lysis was achieved with RIPA buffer. For each IP, samples of the input lysate (I), non-bound flow-through (FT), and bound (B) fractions were analyzed via Coomassie stained SDS-PAGE & Western blot. Anti-NEDD8 polyclonal antibody (16777-1-AP) and Multi-rAb® HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used in the Western blot analysis. The Trap shows efficient IP of endogenous NEDD8 and NEDDylated proteins with low background.

    NEDD8-Trap Agarose (MG-3002A) was used to immunoprecipitate endogenous NEDD8 and NEDDylated proteins from human HEPG2 cells. Lysis was achieved with RIPA buffer. For each IP, samples of the input lysate (I), non-bound flow-through (FT), and bound (B) fractions were analyzed via Coomassie stained SDS-PAGE & Western blot. Anti-NEDD8 polyclonal antibody (16777-1-AP) and Multi-rAb® HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used in the Western blot analysis. The Trap shows efficient IP of endogenous NEDD8 and NEDDylated proteins with low background.

  • NEDD8-Trap Agarose (MG-3002A) was used to immunoprecipitate endogenous NEDD8 and NEDDylated proteins from human HEPG2 cells. Lysis was achieved with standard Lysis buffer. For each IP, samples of the input lysate (I), non-bound flow-through (FT), and bound (B) fractions were analyzed via Coomassie stained SDS-PAGE & Western blot. Anti-NEDD8 polyclonal antibody (16777-1-AP) and Multi-rAb® HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used in the Western blot analysis. The Trap shows efficient IP of endogenous NEDD8 and NEDDylated proteins with low background.

    NEDD8-Trap Agarose (MG-3002A) was used to immunoprecipitate endogenous NEDD8 and NEDDylated proteins from human HEPG2 cells. Lysis was achieved with standard Lysis buffer. For each IP, samples of the input lysate (I), non-bound flow-through (FT), and bound (B) fractions were analyzed via Coomassie stained SDS-PAGE & Western blot. Anti-NEDD8 polyclonal antibody (16777-1-AP) and Multi-rAb® HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used in the Western blot analysis. The Trap shows efficient IP of endogenous NEDD8 and NEDDylated proteins with low background.

  • NEDD8-Trap Agarose (MG-3002A) was used to immunoprecipitate endogenous NEDD8 and NEDDylated proteins from different species / cell lines. Lysis was achieved with standard Lysis buffer. For each IP, samples of the input lysate (I), non-bound flow-through (FT), and bound (B) fractions were analyzed via Western blot. Anti-NEDD8 polyclonal antibody (16777-1-AP) and Multi-rAb® HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used in the Western blot analysis. The Trap shows efficient IP of endogenous NEDD8 and NEDDylated proteins with low background.

    NEDD8-Trap Agarose (MG-3002A) was used to immunoprecipitate endogenous NEDD8 and NEDDylated proteins from different species / cell lines. Lysis was achieved with standard Lysis buffer. For each IP, samples of the input lysate (I), non-bound flow-through (FT), and bound (B) fractions were analyzed via Western blot. Anti-NEDD8 polyclonal antibody (16777-1-AP) and Multi-rAb® HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) were used in the Western blot analysis. The Trap shows efficient IP of endogenous NEDD8 and NEDDylated proteins with low background.

ChromoTek NEDD8-Trap Agarose, Kit for Immunoprecipitation

The ChromoTek NEDD8-Trap Agarose, Kit for Immunoprecipitation consists of an anti-NEDD8 VHH, which is coupled to agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of NEDD8 tagged proteins from cell extracts of various organisms.
Cat No. MG-3002AK

Reactivity

NEDD8

Applications

IP, Co-IP

Host/Type

Alpaca, recombinant VHH

Conjugate

Agarose

Synonyms

Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, Ubiquitin-like protein NEDD8

Formats:  Agarose kit
Size: 

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Product Information

The ChromoTek NEDD8-Trap Agarose, Kit for Immunoprecipitation consists of an anti-NEDD8 VHH, which is coupled to agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of NEDD8 tagged proteins from cell extracts of various organisms.

DescriptionThe NEDD8-Trap Agarose Kit contains NEDD8-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of NEDD8 proteins.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• Stable in harsh buffer conditions

• Suitable for downstream mass spec analysis

• High Specificity

• Low background

ApplicationsIP, Co-IP
Specificity/Target/
Binding Capacity/
Bead concentration50% Slurry
Resin TypeAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution buffer2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5
Wash Buffer Compatibility2M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0.05% SDS, 3-4 M Urea
TypeVHH
ClassRecombinant - Animal free production
HostAlpaca
Affinity350 nM
Compatibility with mass spectrometryThe NEDD8-Trap is optimized for on-bead digestion. For the application note, please click here:
On-bead digest protocol for mass spectrometry
RRIDAB_3740244
Storage Buffer20% Ethanol
Storage ConditionUpon arrival store at +4°C / do not freeze!
ShippingAmbient Temp

Kit components

Component Description
NEDD8-Trap Agarose20 reactions (500 µl)
Lysis bufferOptimized for cytoplasmic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash bufferRemoval of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution
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