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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using NDUFB3 Polyclonal antibody (12358-1-AP)

    WB analysis of human heart using 12358-1-AP (same clone as 12358-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 12358-1-AP (NDUFB3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12358-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using NDUFB3 Polyclonal antibody (12358-1-AP)

    WB analysis of mouse heart using 12358-1-AP (same clone as 12358-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12358-1-AP (NDUFB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12358-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using NDUFB3 Polyclonal antibody (12358-1-AP)

    WB analysis of rat heart using 12358-1-AP (same clone as 12358-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 12358-1-AP (NDUFB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12358-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NDUFB3 Polyclonal antibody (12358-1-AP)

    IHC staining of human liver cancer using 12358-1-AP (same clone as 12358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12358-1-AP (NDUFB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12358-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NDUFB3 Polyclonal antibody (12358-1-AP)

    IHC staining of human liver cancer using 12358-1-AP (same clone as 12358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12358-1-AP (NDUFB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12358-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NDUFB3 Polyclonal antibody (12358-1-AP)

    IHC staining of human prostate cancer using 12358-1-AP (same clone as 12358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12358-1-AP (NDUFB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12358-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NDUFB3 Polyclonal antibody (12358-1-AP)

    IHC staining of human prostate cancer using 12358-1-AP (same clone as 12358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12358-1-AP (NDUFB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12358-1-PBS in a different storage buffer formulation.

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NDUFB3 Polyclonal antibody, PBS Only

NDUFB3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12358-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12358-1-PBS targets NDUFB3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFB3 fusion protein Ag3022 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3, 12kDa
Calculated molecular weight 98 aa, 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC018183
Gene Symbol NDUFB3
Gene ID (NCBI) 4709
RRIDAB_2282633
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43676
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NDUFB3(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3) is also named as CI-B12 and belongs to the complex I NDUFB3 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.