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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using NDUFB3 Polyclonal antibody (12358-1-AP)

    WB analysis of human heart using 12358-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12358-1-AP (NDUFB3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using NDUFB3 Polyclonal antibody (12358-1-AP)

    WB analysis of mouse heart using 12358-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12358-1-AP (NDUFB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using NDUFB3 Polyclonal antibody (12358-1-AP)

    WB analysis of rat heart using 12358-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 12358-1-AP (NDUFB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NDUFB3 Polyclonal antibody (12358-1-AP)

    IHC staining of human liver cancer using 12358-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12358-1-AP (NDUFB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NDUFB3 Polyclonal antibody (12358-1-AP)

    IHC staining of human liver cancer using 12358-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12358-1-AP (NDUFB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NDUFB3 Polyclonal antibody (12358-1-AP)

    IHC staining of human prostate cancer using 12358-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12358-1-AP (NDUFB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NDUFB3 Polyclonal antibody (12358-1-AP)

    IHC staining of human prostate cancer using 12358-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12358-1-AP (NDUFB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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NDUFB3 Polyclonal antibody

NDUFB3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12358-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

B12, CI B12, Complex I B12, NDUFB3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman heart tissue, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12358-1-AP targets NDUFB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFB3 fusion protein Ag3022 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3, 12kDa
Calculated molecular weight 98 aa, 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC018183
Gene Symbol NDUFB3
Gene ID (NCBI) 4709
RRIDAB_2282633
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43676
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDUFB3(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3) is also named as CI-B12 and belongs to the complex I NDUFB3 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.

Protocols

Product Specific Protocols
WB protocol for NDUFB3 antibody 12358-1-APDownload protocol
IHC protocol for NDUFB3 antibody 12358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Hazard Mater

Maternal exposure to E 551 during pregnancy leads to genome-wide DNA methylation changes and metabolic disorders in the livers of pregnant mice and their fetuses

Authors - Yingqi Zhan
View Article