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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFA10 Polyclonal antibody (32947-1-AP)

    WB analysis using 32947-1-AP (same clone as 32947-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32947-1-AP (NDUFA10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NDUFA10 Polyclonal antibody (32947-1-AP)

    WB analysis using 32947-1-AP (same clone as 32947-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32947-1-AP (NDUFA10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NDUFA10 Polyclonal antibody (32947-1-AP)

    IHC staining of human ovary tumor using 32947-1-AP (same clone as 32947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 32947-1-AP (NDUFA10 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using NDUFA10 Polyclonal antibody (32947-1-AP)

    IF Staining of A431 using 32947-1-AP (same clone as 32947-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NDUFA10 antibody (32947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NDUFA10 Polyclonal antibody (32947-1-AP)

    IF Staining of HeLa using 32947-1-AP (same clone as 32947-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFA10 antibody (32947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.

NDUFA10 Polyclonal antibody, PBS Only

NDUFA10 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 32947-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CI-42kD, Complex I-42kD, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial, NADH:Ubiquinone Oxidoreductase Subunit A10, NADH-ubiquinone oxidoreductase 42 kDa subunit

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

32947-1-PBS targets NDUFA10 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag37576

Product name: Recombinant human NDUFA10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-355 aa of BC003417

Sequence: MALRLLKLAATSASARVVAAGAQRVRGIHSSVQCKLRYGMWHFLLGDKASKRLTERSRVITVDGNICTGKGKLAKEIAEKLGFKHFPEAGIHYPDSTTGDGKPLATDYNGNCSLEKFYDDPRSNDGNSYRLQSWLYSSRLLQYSDALEHLLTTGQGVVLERSIFSDFVFLEAMYNQGFIRKQCVDHYNEVKSVTICDYLPPHLVIYIDVPVPEVQRRIQKKGDPHEMKITSAYLQDIENAYKKTFLPEMSEKCEVLQYSAREAQDSKKVVEDIEYLKFDKGPWLKQDNRTLYHLRLLVQDKFEVLNYTSIPIFLPEVTIGAHQTDRVLHQFRELPGRKYSPGYNTEVGDKWIWLK

相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa
Calculated molecular weight 41 kDa
Observed molecular weight40 kDa
GenBank accession numberBC003417
Gene Symbol NDUFA10
Gene ID (NCBI) 4705
RRIDAB_3742801
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDO95299
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NDUFA10 (NADH:ubiquinone oxidoreductase subunit A10) is a subunit of mitochondrial complex I of the electron transport chain, encoding a protein with NADH dehydrogenase and oxidoreductase activity. This protein transfers electrons from NADH to the respiratory chain. Mutations in the NDUFA10 gene are associated with Leigh syndrome, a common mitochondrial energy metabolism disorder in children. Additionally, the expression levels of NDUFA10 are related to the occurrence and prognosis of various diseases. For example, in Alzheimer's disease, its low expression is associated with increased dementia severity. In prostate cancer, low expression of NDUFA10 predicts a poor prognosis.

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