Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, L02 cells, RAW264.7 cells, mouse liver tissue, Jurkat cells, RAW 264.7 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32947-1-AP targets NDUFA10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37576 Product name: Recombinant human NDUFA10 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-355 aa of BC003417 Sequence: MALRLLKLAATSASARVVAAGAQRVRGIHSSVQCKLRYGMWHFLLGDKASKRLTERSRVITVDGNICTGKGKLAKEIAEKLGFKHFPEAGIHYPDSTTGDGKPLATDYNGNCSLEKFYDDPRSNDGNSYRLQSWLYSSRLLQYSDALEHLLTTGQGVVLERSIFSDFVFLEAMYNQGFIRKQCVDHYNEVKSVTICDYLPPHLVIYIDVPVPEVQRRIQKKGDPHEMKITSAYLQDIENAYKKTFLPEMSEKCEVLQYSAREAQDSKKVVEDIEYLKFDKGPWLKQDNRTLYHLRLLVQDKFEVLNYTSIPIFLPEVTIGAHQTDRVLHQFRELPGRKYSPGYNTEVGDKWIWLK 相同性解析による交差性が予測される生物種 |
| Full Name | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa |
| Calculated molecular weight | 41 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC003417 |
| Gene Symbol | NDUFA10 |
| Gene ID (NCBI) | 4705 |
| RRID | AB_3742801 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O95299 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NDUFA10 (NADH:ubiquinone oxidoreductase subunit A10) is a subunit of mitochondrial complex I of the electron transport chain, encoding a protein with NADH dehydrogenase and oxidoreductase activity. This protein transfers electrons from NADH to the respiratory chain. Mutations in the NDUFA10 gene are associated with Leigh syndrome, a common mitochondrial energy metabolism disorder in children. Additionally, the expression levels of NDUFA10 are related to the occurrence and prognosis of various diseases. For example, in Alzheimer's disease, its low expression is associated with increased dementia severity. In prostate cancer, low expression of NDUFA10 predicts a poor prognosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NDUFA10 antibody 32947-1-AP | Download protocol |
| IHC protocol for NDUFA10 antibody 32947-1-AP | Download protocol |
| WB protocol for NDUFA10 antibody 32947-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |